Genetic alterations in potassium transport in L cells
| Autor: | Edward A. Adelberg, Carolyn W. Slayman, I L Miller, J. J. Gargus |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Multidisciplinary
Sodium Cell Mutant Biological Transport Active Biological Transport Potassium transport Biology Molecular biology Kinetics L Cells medicine.anatomical_structure Biochemistry Furosemide Mutation Potassium medicine Efflux Fibroblastic cell Intracellular Transport system Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 75:5589-5593 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.75.11.5589 |
| Popis: | Starting with mutagenized cultures of the mouse fibroblastic cell line LM(TK-), we have selected mutant clones by their ability to grow at 0.2 micrometer K+, a concentration unable to support the growth of the parent cell. The mutants fall into two classes on the basis of their potassium transport properties. Both classes maintain a high intracellular K+ concentration when growing in low-potassium medium, and both are unaltered in the ouabain-sensitive Na/K pump. One class shows an increased activity of a ouabain-resistant, furosemide-sensitive K+ transport system; the other class shows a decreased activity of a specific component of K+ efflux. |
| Databáze: | OpenAIRE |
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