Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)
| Autor: | Maria Júlia Barbosa Bezerra, Celso Shiniti Nagano, Thais Thatiane dos Santos Souza, Maurício Fraga van Tilburg, Luciana Magalhães Melo, Vicente José de Figueirêdo Freitas, Luciana Diniz Rola, Arlindo A. Moura, José Maurício Barbanti Duarte |
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| Přispěvatelé: | Univ Estadual Ceara, Univ Fed Ceara, Universidade Estadual Paulista (Unesp), Univ Ctr Fametro |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-25T11:23:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (FUNCAP) The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P> 0.05) was observed between small/medium and large follicles: 49.2 +/- 22.8 and 56.7 +/- 27.4 mu g/mu l, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P> 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer. Univ Estadual Ceara, Fac Vet, Av Dr Silas Munguba 1700, BR-60714903 Fortaleza, Ceara, Brazil Univ Fed Ceara, Dept Anim Sci, Fortaleza, Ceara, Brazil Univ Estadual Ceara, Hlth Sci Ctr, Fortaleza, Ceara, Brazil Univ Fed Ceara, Lab Mass Spectrometry, Fortaleza, Ceara, Brazil State Univ Sao Paulo, Dept Anim Sci, Jaboticabal, Brazil Univ Ctr Fametro, Mol Genet Res Unit, Fortaleza, Ceara, Brazil State Univ Sao Paulo, Dept Anim Sci, Jaboticabal, Brazil CNPq: 461330/2014-8 (FUNCAP): CI3-0093-0005580100/14 |
| Databáze: | OpenAIRE |
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