Reductive evolution of virulence repertoire to drive the divergence between community- and hospital-associated methicillin-resistant Staphylococcus aureus of the ST1 lineage

Autor: Ana Tereza Ribeiro de Vasconcelos, Marisa Fabiana Nicolás, Patricia Martins-Simoes, Nicholas Costa Barroso Lima, William Mouton, Paul J. Planet, Rangel C. Souza, Marina Farrel Côrtes, Michèle Bes, Ana Maria Nunes Botelho, André E. R. Soares, Agnes Marie Sá Figueiredo, Paula Terra Bandeira, Luiz Gonzaga Paula de Almeida, Frédéric Laurent, Cédric Badiou
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Microbiology (medical)
Methicillin-Resistant Staphylococcus aureus
Lineage (genetic)
Genotype
Virulence Factors
Immunology
Virulence
evolution of pathogenicity
Infectious and parasitic diseases
RC109-216
comparative genomics
Biology
medicine.disease_cause
Microbiology
Divergence
Evolution
Molecular
03 medical and health sciences
medicine
Humans
Phylogeny
030304 developmental biology
Comparative genomics
Genetics
Whole genome sequencing
0303 health sciences
whole genome sequencing
Cross Infection
030306 microbiology
Repertoire
Bayes Theorem
Genomics
biochemical phenomena
metabolism
and nutrition
Staphylococcal Infections
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Community-Acquired Infections
Infectious Diseases
Staphylococcus aureus
ST1-SCCmecIV
Parasitology
Genome
Bacterial
Research Article
Research Paper
evolution of virulence
Zdroj: Virulence
article-version (VoR) Version of Record
Virulence, Vol 12, Iss 1, Pp 951-967 (2021)
ISSN: 2150-5608
2150-5594
Popis: Methicillin-resistant Staphylococcus aureus (MRSA) of the ST1-SCCmecIV lineage has been associated with community-acquired (CA) infections in North America and Australia. In Brazil, multi-drug resistant ST1-SCCmecIV MRSA has emerged in hospital-associated (HA) diseases in Rio de Janeiro. To understand these epidemiological differences, genomic and phylogenetic analyses were performed. In addition, virulence assays were done for representative CA – and HA-MRSA strains. Despite the conservation of the virulence repertoire, some genes were missing in Brazilian ST1-SCCmecIV including lukSF-PV, fnbB, and several superantigen-encoded genes. Additionally, CA-MRSA lost the splDE while HA-MRSA strains conserved the complete operon. Most of these variable genes were located in mobile genetic elements (MGE). However, conservation and maintenance of MGEs were often observed despite the absence of their associated virulence markers. A Bayesian phylogenetic tree revealed the occurrence of more than one entrance of ST1 strains in Rio de Janeiro. The tree shape and chronology allowed us to infer that the hospital-associated ST1-SCCmecIV from Brazil and the community-acquired USA400 from North America are not closely related and that they might have originated from different MSSA strains that independently acquired SCCmecIV cassettes. As expected, representatives of ST1 strains from Brazil showed lower cytotoxicity and a greater ability to survive inside human host cells. We suggest that Brazilian ST1-SCCmecIV strains have adapted to the hospital setting by reducing virulence and gaining the ability to persist and survive inside host cells. Possibly, these evolutionary strategies may balance the biologic cost of retaining multiple antibiotic resistance genes.
Databáze: OpenAIRE
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