Electroacupuncture induces acute changes in cerebral cortical miRNA profile, improves cerebral blood flow and alleviates neurological deficits in a rat model of stroke
Autor: | Wen-Bo Li, Wei Jiang, Hai-Zhen Zheng, Han-Tong Hu, Xiao-Feng Zhao, Xue-min Shi, Pan-Gong Liu, Jing Du, Li-Dan Chang |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Electroacupuncture middle cerebral artery occlusion medicine.medical_treatment Rat model cerebral blood flow Neiguan (PC6) Stimulation lcsh:RC346-429 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience Internal medicine microRNA electroacupuncture medicine nerve regeneration stroke miRNA Renzhong (GV26) neural regeneration lcsh:Neurology. Diseases of the nervous system business.industry Cell growth Vascular endothelial growth factor 030104 developmental biology Endocrinology chemistry Cerebral blood flow Signal transduction business Neuroscience 030217 neurology & neurosurgery Research Article |
Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 11, Iss 12, Pp 1940-1950 (2016) |
ISSN: | 1673-5374 |
Popis: | Electroacupuncture has been shown to improve cerebral blood flow in animal models of stroke. However, it is unclear whether electroacupuncture alters miRNA expression in the cortex. In this study, we examined changes in the cerebral cortical miRNA profile, cerebral blood flow and neurological function induced by electroacupuncture in a rat model of stroke. Electroacupuncture was performed at Renzhong (GV26) and Neiguan (PC6), with a frequency of 2 Hz, continuous wave, current intensity of 3.0 mA, and stimulation time of 1 minute. Electroacupuncture increased cerebral blood flow and alleviated neurological impairment in the rats. miRNA microarray profiling revealed that the vascular endothelial growth factor signaling pathway, which links cell proliferation with stroke, was most significantly affected by electroacupuncture. Electroacupuncture induced changes in expression of rno-miR-206-3p, rno-miR-3473, rno-miR-6216 and rno-miR-494-3p, and these changes were confirmed by quantitative real-time polymerase chain reaction. Our findings suggest that changes in cell proliferation-associated miRNA expression induced by electroacupuncture might be associated with the improved cerebral blood supply and functional recovery following stroke. |
Databáze: | OpenAIRE |
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