Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast
| Autor: | Morgan R. Schroeder, Peter Baumann, Lili Pan, Rutendo F. Sigauke, Diego J. Páez-Moscoso, Wen Tang |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Telomerase Science Protein subunit Molecular Sequence Data General Physics and Astronomy General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Schizosaccharomyces Telomerase reverse transcriptase Amino Acid Sequence lcsh:Science Multidisciplinary 030102 biochemistry & molecular biology biology Chemistry Fungal genetics RNA RNA Fungal General Chemistry Telomere biology.organism_classification Cell biology 030104 developmental biology RNA Recognition Motif Proteins lcsh:Q Schizosaccharomyces pombe Proteins Sequence Alignment Protein Binding |
| Zdroj: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018) |
| ISSN: | 2041-1723 |
| Popis: | Telomerase reverse transcriptase (TERT) and the non-coding telomerase RNA subunit (TR) constitute the core of telomerase. Here we now report that the putative F-box protein Pof8 is also a constitutive component of active telomerase in fission yeast. Pof8 functions in a hierarchical assembly pathway by promoting the binding of the Lsm2-8 complex to telomerase RNA, which in turn promotes binding of the catalytic subunit. Loss of Pof8 reduces TER1 stability, causes a severe assembly defect, and results in critically short telomeres. Structure profile searches identified similarities between Pof8 and telomerase subunits from ciliated protozoa, making Pof8 next to TERT the most widely conserved telomerase subunits identified to date. The core of the telomerase holoenzyme is composed of the reverse transcriptase and the non-coding RNA template. Here the authors identify Pof8 as a new constitutive component of fission yeast telomerase, loss of which causes telomerase assembly defects and critically short telomeres. |
| Databáze: | OpenAIRE |
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