Novel axolotl cardiac function analysis method using magnetic resonance imaging
| Autor: | Holger Grüll, Pedro Gomes Sanches, Wolter L. de Graaf, Roel C. op ‘t Veld, Gustav J. Strijkers |
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| Přispěvatelé: | Amsterdam Cardiovascular Sciences, Amsterdam Movement Sciences, Biomedical Engineering and Physics, ACS - Heart failure & arrhythmias, ACS - Atherosclerosis & ischemic syndromes |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cardiac output Physiology lcsh:Medicine Cardiovascular Physiology Diagnostic Radiology Magnetic Resonance Imaging/methods 0302 clinical medicine Heart Rate Anesthesiology Heart Regeneration Medicine and Health Sciences Morphogenesis Anesthesia lcsh:Science GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Multidisciplinary Ejection fraction medicine.diagnostic_test Pharmaceutics Radiology and Imaging Ultrasound Heart Stroke volume Heart/diagnostic imaging Magnetic Resonance Imaging Body Fluids Blood Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Female Anatomy Research Article Cardiac function curve Imaging Techniques Cardiac Ventricles Cardiology Research and Analysis Methods 03 medical and health sciences All institutes and research themes of the Radboud University Medical Center Drug Therapy Cardiac magnetic resonance imaging Diagnostic Medicine Heart rate medicine Regeneration Animals business.industry lcsh:R Biology and Life Sciences Magnetic resonance imaging Ambystoma mexicanum 030104 developmental biology Cardiovascular Anatomy lcsh:Q Ambystoma mexicanum/physiology business Nuclear medicine Organism Development 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | PLoS One, 12, 8 PLoS ONE, Vol 12, Iss 8, p e0183446 (2017) PLoS One, 12 PLoS ONE, 12(8):e0183446. Public Library of Science PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0183446 |
| Popis: | The salamander axolotl is capable of complete regeneration of amputated heart tissue. However, non-invasive imaging tools for assessing its cardiac function were so far not employed. In this study, cardiac magnetic resonance imaging is introduced as a noninvasive technique to image heart function of axolotls. Three axolotls were imaged with magnetic resonance imaging using a retrospectively gated Fast Low Angle Shot cine sequence. Within one scanning session the axolotl heart was imaged three times in all planes, consecutively. Heart rate, ejection fraction, stroke volume and cardiac output were calculated using three techniques: (1) combined long-axis, (2) short-axis series, and (3) ultrasound (control for heart rate only). All values are presented as mean ± standard deviation. Heart rate (beats per minute) among different animals was 32.2±6.0 (long axis), 30.4±5.5 (short axis) and 32.7±4.9 (ultrasound) and statistically similar regardless of the imaging method (p > 0.05). Ejection fraction (%) was 59.6±10.8 (long axis) and 48.1±11.3 (short axis) and it differed significantly (p = 0.019). Stroke volume (μl/beat) was 133.7±33.7 (long axis) and 93.2±31.2 (short axis), also differed significantly (p = 0.015). Calculations were consistent among the animals and over three repeated measurements. The heart rate varied depending on depth of anaesthesia. We described a new method for defining and imaging the anatomical planes of the axolotl heart and propose one of our techniques (long axis analysis) may prove useful in defining cardiac function in regenerating axolotl hearts. |
| Databáze: | OpenAIRE |
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