Retrotransposon molecular markers resolve cocoyam (Xanthosoma sagittifolium) and taro (Colocasia esculenta) by type and variety
| Autor: | Ruslan Kalendar, Oumar Doungous, Amayana Adiobo, Alan H. Schulman |
|---|---|
| Přispěvatelé: | Institute of Biotechnology, Alan Schulman / Principal Investigator, Viikki Plant Science Centre (ViPS) |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Germplasm 0303 health sciences Genetic diversity biology Xanthosoma 1184 Genetics developmental biology physiology Retrotransposon Plant Science Horticulture Xanthosoma sagittifolium biology.organism_classification 01 natural sciences Colocasia esculenta 03 medical and health sciences Botany Genetics Genetic variability Agronomy and Crop Science 1183 Plant biology microbiology virology Colocasia 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Euphytica. 206:541-554 |
| ISSN: | 1573-5060 0014-2336 |
| Popis: | Retrotransposon-based molecular markers were applied for the first time within the genera Xanthosoma and Colocasia to assess intraspecific variability among 27 accessions of cocoyam (Xanthosoma sagittifolium) and taro (Colocasia esulenta). Over 16 distinct retrotransposon fragments were isolated, sequenced, and LTR primers were designed to obtain Inter-Retrotransposon Amplified Polymorphism (IRAP) fingerprints. The set of six polymorphic LTR primers yielded 433 reproducible bands across a set of 20 X. sagittifolium samples. Out of the 433 bands, 400 fragments (92%) were polymorphic. In seven C. esculenta accessions, the six primers amplified a total of 354 reproducible, informative data points, of which 285 (80.5%) were polymorphic. Data concerning the number of polymorphic bands and Shannon’s index in X. sagittifolium accessions suggest that retrotransposon activity continued after Xanthosoma speciation. Cluster analysis placed all the accessions in two groups according to their species delimitation. The accessions of X. sagittifolium were further divided into two subgroups corresponding to their ploidy level. Moreover, our results showed that the genetic variability accessed by IRAP markers allows separation of X. sagittifolium and C. esculenta accessions according to their type and botanical variety respectively. These data provide a basis for better germplasm management, future systematic studies and genetic improvement, as well as for exploration of the role of retrotransposons in cocoyam and taro polyploid formation and genome dynamics. |
| Databáze: | OpenAIRE |
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