Molecular characterization of a cDNA encoding DRE-binding transcription factor from dehydration-treated fibrous roots of sweetpotato
| Autor: | Sun-Hwa Ryu, Jae-Wook Bang, Sang-Soo Kwak, Wan-Keun Song, Yun-Hee Kim, Haeng-Soon Lee, Suk-Yoon Kwon, Kyoung-Sil Yang, Kee-Yeun Kim |
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| Rok vydání: | 2008 |
| Předmět: |
DNA
Complementary Physiology Molecular Sequence Data Fibrous root system Plant Science Biology Plant Roots chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Complementary DNA Genetics Amino Acid Sequence Ipomoea batatas Gene Transcription factor Abscisic acid Phylogeny Plant Proteins Messenger RNA Base Sequence Reverse Transcriptase Polymerase Chain Reaction cDNA library Abiotic stress Water food and beverages Molecular biology Plant Leaves Biochemistry chemistry Sequence Alignment Abscisic Acid Transcription Factors |
| Zdroj: | Plant Physiology and Biochemistry. 46:196-204 |
| ISSN: | 0981-9428 |
| Popis: | A new dehydration responsive element-binding (DREB) protein gene encoding for an AP2/EREBP-type transcription factor was isolated by screening of the cDNA library for dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). Its cDNA (referred to as swDREB1) fragment of 1206 bp was sequenced from, which a 257 amino acid residue protein was deduced with a predicted molecular weight of 28.17 kDa. A search of the protein BLAST database revealed that this protein can be classified as a typical member of a DREB subfamily. RT-PCR and northern analyses revealed diverse expression patterns of the swDREB1 gene in various tissues of intact sweetpotato plant, and in leaves and fibrous roots exposed to different stresses. The swDREB1 gene was highly expressed in stems and tuberous roots. In fibrous roots, its mRNA accumulation profiles clearly showed strong expression under various abiotic stress conditions such as dehydration, chilling, salt, methyl viologen (MV), and cadmium (Cd) treatment, whereas it did not respond to abscisic acid (ABA) or copper (Cu) treatment. The above results indicate that swDREB1 may be involved in the process of the plant response to diverse abiotic stresses through an ABA-independent pathway. |
| Databáze: | OpenAIRE |
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