Patients with oral squamous cell carcinoma are characterized by increased frequency of suppressive regulatory T cells in the blood and tumor microenvironment
| Autor: | Edgard Jose Franco de Melo, Maura Rosane Valério Ikoma, Tatiana Salles de Souza Malaspina, Gustavo Pompermaier Garlet, Karen A. Cavassani, Maria Renata Sales Nogueira Costa, Luciana Benevides, José Humberto Damante, Thaís Helena Gasparoto, João Santana da Silva, Ana Paula Campanelli |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_treatment Immunology chemical and pharmacologic phenomena T-Lymphocytes Regulatory Immunophenotyping Interleukin 21 Immune system Antigen Antigens CD T-Lymphocyte Subsets medicine Humans Immunology and Allergy IL-2 receptor Aged Cell Proliferation Aged 80 and over Tumor microenvironment business.industry FOXP3 Forkhead Transcription Factors hemic and immune systems Immunotherapy Middle Aged Antigens Differentiation stomatognathic diseases Oncology Carcinoma Squamous Cell Cytokines Female Mouth Neoplasms Tumor Escape business NEOPLASIAS BUCAIS (IMUNOLOGIA) |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Oral squamous cell carcinoma (OSCC) is a cancerous lesion with high incidence worldwide. The immunoregulatory events leading to OSCC persistence remain to be elucidated. Our hypothesis is that regulatory T cells (Tregs) are important to obstruct antitumor immune responses in patients with OSCC. In the present study, we investigated the frequency, phenotype, and activity of Tregs from blood and lesions of patients with OSCC. Our data showed that80% of CD4(+)CD25(+) T cells isolated from PBMC and tumor sites express FoxP3. Also, these cells express surface Treg markers, such as GITR, CD45RO, CD69, LAP, CTLA-4, CCR4, and IL-10. Purified CD4(+)CD25(+) T cells exhibited stronger suppressive activity inhibiting allogeneic T-cell proliferation and IFN-gamma production when compared with CD4(+)CD25(+) T cells isolated from healthy individuals. Interestingly, approximately 25% of CD4(+)CD25(-) T cells of PBMC from patients also expressed FoxP3 and, although these cells weakly suppress allogeneic T cells proliferative response, they inhibited IFN-gamma and induced IL-10 and TGF-beta secretion in these co-cultures. Thus, our data show that Treg cells are present in OSCC lesions and PBMC, and these cells appear to suppress immune responses both systemically and in the tumor microenvironment. |
| Databáze: | OpenAIRE |
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