Evaluation of recombinant fructose-1,6-bisphosphate aldolase ELISA test for the diagnosis of Schistosoma japonicum in water buffaloes
| Autor: | Kin Mu Lee, John Chin Tsaihong, Ping Chin Fan, Po Ching Cheng, Shih Yi Peng |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
China
DNA Complementary Fructose 1 6-bisphosphate Buffaloes Molecular Sequence Data Antibodies Helminth Enzyme-Linked Immunosorbent Assay Biology Schistosoma japonicum law.invention chemistry.chemical_compound Immune system Antigen law Fructose-Bisphosphate Aldolase parasitic diseases Animals Amino Acid Sequence Cloning Molecular chemistry.chemical_classification Base Sequence General Veterinary Aldolase A Gene Amplification food and beverages biology.organism_classification Molecular biology Recombinant Proteins Enzyme chemistry Schistosomiasis japonica biology.protein Recombinant DNA Antibody |
| Zdroj: | Research in Veterinary Science. 85:527-533 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/j.rvsc.2008.02.005 |
| Popis: | Fructose-1,6-bisphosphate aldolase (FBPA) is an ubiquitous enzyme essential for glycolysis, gluconeogenesis and the Calvin cycle. It has been demonstrated to induce immune responses and to be useful in the immunodiagnosis of malaria. In this study, FBPA was cloned from the adult worms of Schistosoma japonicum and tested as an antigen for the diagnosis of S. japonicum infection in water buffaloes. Enzyme-linked immunosorbent assay (ELISA) was performed on the sera from 32 infected water buffaloes and 20 negative controls using the recombinant FBPA protein or soluble worm antigen preparation (SWAP) as an antigen. The OD cut-off values were determined to be 0.57 with 100% specificity and 100% sensitivity for the FBPA ELISA and 1.13 with 93.8% specificity and 95.0% sensitivity for the SWAP ELISA. These findings indicate that the recombinant FBPA of S. japonicum should be an useful diagnostic tool for the detection of antibodies against S. japonicum. |
| Databáze: | OpenAIRE |
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