Intestinal colonization by Candida albicans alters inflammatory responses in Bruton's tyrosine kinase-deficient mice

Autor: Strijbis, Karin, Yilmaz, Omer H, Dougan, Stephanie K, Esteban, Alexandre, Gröne, Andrea, Kumamoto, Carol A, Ploegh, Hidde L, Strategic Infection Biology, PB SIB, LS Infectiebiologie (Bacteriologie), I&I SIB2, LS Pathologie, Sub Cellular Protein Chemistry
Přispěvatelé: Strategic Infection Biology, PB SIB, LS Infectiebiologie (Bacteriologie), I&I SIB2, LS Pathologie, Sub Cellular Protein Chemistry
Rok vydání: 2014
Předmět:
medicine.medical_treatment
Pathology and Laboratory Medicine
Biochemistry
Immune Receptors
immune system diseases
Transforming Growth Factor beta
Animal Cells
hemic and lymphatic diseases
Candida albicans
Agammaglobulinaemia Tyrosine Kinase
Medicine and Health Sciences
Intestinal Mucosa
Immune Response
Mice
Knockout
Fungal Pathogens
Gastrointestinal tract
Multidisciplinary
Immune System Proteins
biology
Dextran Sulfate
Interleukin-17
Candidiasis
Fungal Diseases
Organ Size
Protein-Tyrosine Kinases
Colitis
Immunohistochemistry
Corpus albicans
Interleukin-10
Intestines
medicine.anatomical_structure
Cytokine
Infectious Diseases
Medical Microbiology
Host-Pathogen Interactions
Medicine
Cytokines
Inflammation Mediators
Cellular Types
Pathogens
Research Article
Colon
Science
Immune Cells
Immunology
Spleen
Immunopathology
Gastroenterology and Hepatology
Opportunistic Infections
Microbiology
medicine
Bruton's tyrosine kinase
Animals
Humans
Microbial Pathogens
Macrophages
Soft Tissue Infections
Inflammatory Bowel Disease
Wild type
Immunity
Biology and Life Sciences
Proteins
Cell Biology
biology.organism_classification
medicine.disease
digestive system diseases
Yeast Infections
biology.protein
Clinical Immunology
Pattern Recognition Receptors
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 11, p e112472 (2014)
PLoS One, 9(11). Public Library of Science
ISSN: 1932-6203
Popis: The commensal yeast Candida albicans is part of the human intestinal microflora and is considered a "pathobiont", a resident microbe with pathogenic potential yet harmless under normal conditions. The aim of this study was to investigate the effect of C. albicans on inflammation of the intestinal tract and the role of Bruton's tyrosine kinase (Btk). Btk is an enzyme that modulates downstream signaling of multiple receptors involved in innate and adaptive immunity, including the major anti-fungal receptor Dectin-1. Colitis was induced in wild type and Btk-/- mice by treatment with dextran sodium sulfate (DSS) and the gastrointestinal tract of selected treatment groups were then colonized with C. albicans. Colonization by C. albicans neither dampened nor exacerbated inflammation in wild type mice, but colon length and spleen weight were improved in Btk-deficient mice colonized with C. albicans. Neutrophil infiltration was comparable between wild type and Btk-/- mice, but the knockout mice displayed severely reduced numbers of macrophages in the colon during both DSS and DSS/Candida treatment. Smaller numbers and reduced responsiveness of Btk-/- macrophages might partially explain the improved colon length of Btk-/- mice as a result of Candida colonization. Surprisingly, DSS/Candida-treated Btk-/- animals had higher levels of certain pro-inflammatory cytokines and levels of the anti-inflammatory cytokine TGF-β were reduced compared to wild type. A clustering and correlation analysis showed that for wild type animals, spleen TGF-β and colon IL-10 and for Btk-/- spleen and colon levels of IL-17A best correlated with the inflammatory parameters. We conclude that in Btk-/- immunocompromised animals, colonization of the gastrointestinal tract by the commensal yeast C. albicans alters inflammatory symptoms associated with colitis.
Databáze: OpenAIRE
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