Cytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa) and avocado (Persea americana); evidence for related enzymes with different activities
| Autor: | Rolf E. Christoffersen, Sze-Mei C. Lau, D. L. Hallahan, Glenn W. Dawson, Patricia A. Harder, D. W. M. Smiley, Daniel P. O'Keefe, John A. Pickett |
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| Rok vydání: | 1994 |
| Předmět: |
Persea
biology Cytochrome Terpenes Acyclic Monoterpenes Monoterpene Biophysics Nepeta racemosa food and beverages Cytochrome P450 Plants biology.organism_classification Biochemistry Gas Chromatography-Mass Spectrometry Hydroxylation chemistry.chemical_compound Cytochrome P-450 Enzyme System chemistry Botany biology.protein Nerol Oxidation-Reduction Molecular Biology Geraniol |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1201:94-100 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(94)90156-2 |
| Popis: | A cytochrome P-450 present in ripening avocado (Persea americana) fruit mesocarp (CYP71A1) had previously been shown to metabolize the monoterpenoids nerol and geraniol (Hallahan et al. (1992) Plant Physiol. 98, 1290-1297). Using DNA encoding CYP71A1 as a hybridization probe, we have shown by Southern analysis that a related gene is present in the catmint, Nepeta racemosa. RNA blot analysis, together with Western analysis of catmint leaf polypeptides using avocado cyt P-450 antiserum, showed that a closely related gene is expressed in catmint leaves. Cytochrome P-450 in catmint microsomes catalysed the specific hydroxylation of nerol and geraniol at C-10, whereas avocado CYP71A1, in either avocado microsomes or heterologously expressed in yeast, catalysed 2,3- or 6,7-epoxidation of these substrates. These results suggest that orthologous genes of the CYP71 family are expressed in these two plant species, but catalyse dissimilar reactions with monoterpenoid substrates. |
| Databáze: | OpenAIRE |
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