Evaluation of Streptococcus mutans adhesion to fluoride varnishes and subsequent change in biofilm accumulation and acidogenicity
| Autor: | Ngoc Phuong Thanh Chau, Ji-Eun Jung, Jae-Gyu Jeon, Santosh Pandit |
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| Rok vydání: | 2014 |
| Předmět: |
Surface Properties
Polyurethanes Varnish Bacterial Adhesion Microbiology Streptococcus mutans chemistry.chemical_compound Coated Materials Biocompatible Bifluorid 12 Materials Testing Humans Fluorides Topical Biomass General Dentistry Colony-forming unit Microscopy Confocal biology Polysaccharides Bacterial Fluoride varnish Biofilm Adhesion Hydrogen-Ion Concentration Silanes biochemical phenomena metabolism and nutrition biology.organism_classification Bacterial Load Cariostatic Agents Culture Media Calcium Fluoride Drug Combinations stomatognathic diseases Durapatite chemistry Biofilms visual_art visual_art.visual_art_medium Sodium Fluoride Acids Fluoride |
| Zdroj: | Journal of Dentistry. 42:726-734 |
| ISSN: | 0300-5712 |
| Popis: | Objectives The aim of this study was to evaluate Streptococcus mutans adhesion to fluoride varnishes and subsequent change in biofilm accumulation and acidogenicity. Methods After producing fluoride varnish-coated hydroxyapatite discs using Fluor Protector (FP), Bifluorid 12 (BIF), Cavity Shield (CASH), or Flor-Opal Varnish White (FO), S. mutans biofilms were formed on the discs. To assess S. mutans adhesion to the discs, 4-h-old biofilms were analysed. To investigate the change in biofilm accumulation during subsequent biofilm formation, the biomass, colony forming units (CFU), and water-insoluble extracellular polysaccharides (EP) of 46-, 70-, and 94-h-old biofilms were analysed. To investigate the change in acidogenicity, pH values of the culture medium were determined during the experimental period. The amount of fluoride in the culture medium was also determined during the experimental period. Results BIF, CASH, and FO affected S. mutans adhesion (67–98% reduction) and subsequent biofilm accumulation in 46-, 70-, and 94-h-old biofilms. However, the reducing effect of the fluoride varnishes on the biomass, CFU count, water-insoluble EP amount, and acid production rate of the biofilms decreased as the biofilm age increased. These results may be related to the fluoride-release pattern of the fluoride varnishes. Of the fluoride varnishes tested, FO showed the highest reducing effect against the bacterial adhesion and subsequent biofilm accumulation. Conclusions Our findings suggest that if the results of these experiments are extrapolable to the in vivo situation, then reduced clinical benefit of using fluoride varnishes may occur with time. Clinical significance Fluoride varnish application can affect cariogenic biofilm formation but the anti-biofilm activity may be reduced with time. |
| Databáze: | OpenAIRE |
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