The Autophagy-Related Organelle Autophagoproteasome Is Suppressed within Ischemic Penumbra
Autor: | Carla L. Busceti, Paola Lenzi, Francesco Fornai, Domenico Bucci, Rosangela Ferese, Francesca Biagioni, Stefano Puglisi-Allegra, Federica Mastroiacovo, Alessandro Frati, Ferdinando Nicoletti, Larisa Ryskalin |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Male
Autophagy vacuoles Compartmentalization Heat shock protein 70 (HSP70) LC3 P20S Proteasome Stoichiometry Ultrastruc-tural morphometry Animals Biomarkers HSP70 Heat-Shock Proteins Mice Microtubule-Associated Proteins Autophagosomes Autophagy Ischemic Stroke QH301-705.5 Cell Vacuole Biology Catalysis Article Inorganic Chemistry autophagy vacuoles Organelle medicine heat shock protein 70 (HSP70) Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Penumbra Organic Chemistry General Medicine Compartmentalization (psychology) compartmentalization Computer Science Applications Hsp70 Cell biology stoichiometry ultrastructural morphometry Chemistry medicine.anatomical_structure proteasome |
Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 19 International Journal of Molecular Sciences, Vol 22, Iss 10364, p 10364 (2021) |
ISSN: | 1422-0067 |
Popis: | The peri-infarct region, which surrounds the irreversible ischemic stroke area is named ischemic penumbra. This term emphasizes the borderline conditions for neurons placed within such a critical region. Area penumbra separates the ischemic core, where frank cell loss occurs, from the surrounding healthy brain tissue. Within such a brain region, nervous matter, and mostly neurons are impaired concerning metabolic conditions. The classic biochemical marker, which reliably marks area penumbra is the over-expression of the heat shock protein 70 (HSP70). However, other proteins related to cell clearing pathways are modified within area penumbra. Among these, autophagy proteins like LC3 increase in a way, which recapitulates Hsp70. In contrast, components, such as P20S, markedly decrease. Despite apparent discrepancies, the present study indicates remarkable overlapping between LC3 and P20S redistribution within area penumbra. In fact, the amount of both proteins is markedly reduced within vacuoles. Specifically, a massive loss of LC3 + P20S immuno-positive vacuoles (autophagoproteasomes) is reported here. This represents the most relevant sub-cellular alteration here described in cell clearing pathways within area penumbra. The functional significance of these findings remains to be determined and it will take a novel experimental stream to decipher the fine-tuning of such a phenomenon. |
Databáze: | OpenAIRE |
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