Counter-current distribution of cells
| Autor: | P.Å. Albertsson, G.D. Baird |
|---|---|
| Rok vydání: | 1962 |
| Předmět: |
Aqueous solution
Sodium Histological Techniques chemistry.chemical_element Context (language use) Cell Biology Fractionation Polyethylene glycol Biology Diploidy Chemistry Techniques Analytical chemistry.chemical_compound chemistry Biochemistry Chemical engineering Phase (matter) Partition (number theory) Particle |
| Zdroj: | Experimental Cell Research. 28:296-322 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(62)90285-9 |
| Popis: | 1. 1. The partition behavior of a variety of cells has been examined in phase systems obtained by mixing aqueous solutions of dextran and polyethylene glycol in appropriate proportions. The effect of increasing concentrations of sodium chloride on the behavior of the cells has also been studied. 2. 2. The cells employed in this work have been viewed as model particles, whose behavior in these systems could be taken as representative of that of other types of large biological particle. Bacterial, algal and yeast cells, and also erythrocytes, were selected as appropriate model particles in this context. 3. 3. Work has been presented which demonstrates that the cells behave in a fashion which approximates to that which is theoretically ideal when they are subjected to counter-current distribution of the type due to Craig [4]. A fractionation of a mixture of cell populations, each of which has a differing partition behavior in the phase system in question, is therefore possible. 4. 4. Finally, a method has been described whereby the efficiency of the fractionation of cell mixtures by the counter-current procedure can be increased. This method involves the development in the distribution chain of a gradient of sodium chloride concentration. |
| Databáze: | OpenAIRE |
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