Development of a single-step duplex RT-PCR detecting different forms of ret activation, and identification of the third form of in vivo ret activation in human papillary thyroid carcinoma
| Autor: | Ernest L. Mazzaferri, Patricia A. Smanik, Sissy M. Jhiang |
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| Rok vydání: | 1994 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities endocrine system Cancer Research endocrine system diseases Molecular Sequence Data Biology Receptor tyrosine kinase Thyroid carcinoma Rapid amplification of cDNA ends Proto-Oncogene Proteins medicine Drosophila Proteins Humans Amino Acid Sequence RNA Neoplasm Thyroid Neoplasms Thyroid cancer Gene Rearrangement Base Sequence Oncogene Carcinoma Proto-Oncogene Proteins c-ret Receptor Protein-Tyrosine Kinases Oncogenes Gene rearrangement medicine.disease Gene Expression Regulation Neoplastic Real-time polymerase chain reaction Genes Oncology Cancer research biology.protein Tyrosine kinase |
| Zdroj: | Cancer Letters. 78:69-76 |
| ISSN: | 0304-3835 |
| Popis: | In up to 25% of human papillary thyroid carcinomas (PCs), the oncogenic activation of ret results from the fusion of its tyrosine kinase domain with different unlinked amino-terminal sequences. Activation of this oncogene may be of prognostic significance in patients with PC. To screen for ret activation in archival paraffin-embedded tumors, we have developed a single-step duplex RT-PCR to detect different forms of ret activation despite different chimeric transcripts being expressed. Furthermore, we report a third type of ret oncogenic activation, named ret/PTC3, identified by using this novel method followed by rapid amplification of cDNA ends (5'-RACE) cloning. |
| Databáze: | OpenAIRE |
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