Alterations in lipid profile upon uterine fibroids and its recurrence
| Autor: | Alisa O. Tokareva, Vladimir Frankevich, Natalia L. Starodubtseva, Konstantin Chingin, Irena F. Kozachenko, Vitaliy Chagovets, Narine M. Tonoyan, Leyla V. Adamyan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
medicine.medical_specialty Uterine fibroids Science Predictive markers Gastroenterology Asymptomatic Article Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Prognostic markers 0302 clinical medicine Internal medicine Lipidomics Blood plasma medicine Humans Chromatography High Pressure Liquid 030219 obstetrics & reproductive medicine Multidisciplinary medicine.diagnostic_test Molecular medicine Leiomyoma Cholesterol business.industry Myometrium Diagnostic markers Middle Aged medicine.disease Lipids chemistry 030220 oncology & carcinogenesis Uterine Neoplasms Medicine Female medicine.symptom Neoplasm Recurrence Local Sphingomyelin business Lipid profile Follow-Up Studies |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Uterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. MS analysis of lipid levels in the myometrium tissues, fibroids tissues and blood plasma samples was carried out on 66 patients, including 35 patients with first-time diagnosed UF and 31 patients with RUF. The control group consisted of 15 patients who underwent surgical treatment for the intrauterine septum. Fibroids and myometrium tissue samples were analyzed using direct MS approach. Blood plasma samples were analyzed using high performance liquid chromatography hyphened with mass spectrometry (HPLC/MS). MS data were processed by discriminant analysis with projection into latent structures (OPLS-DA). Significant differences were found between the first-time UF, RUF and control group in the levels of lipids involved in the metabolism of glycerophospholipids, sphingolipids, lipids with an ether bond, triglycerides and fatty acids. Significant differences between the control group and the groups with UF and RUF were found in the blood plasma levels of cholesterol esters, triacylglycerols, (lyso) phosphatidylcholines and sphingomyelins. Significant differences between the UF and RUF groups were found in the blood plasma levels of cholesterol esters, phosphotidylcholines, sphingomyelins and triacylglycerols. Diagnostic models based on the selected differential lipids using logistic regression showed sensitivity and specificity of 88% and 86% for the diagnosis of first-time UF and 95% and 79% for RUF, accordingly. This study confirms the involvement of lipids in the pathogenesis of uterine fibroids. A diagnostically significant panel of differential lipid species has been identified for the diagnosis of UF and RUF by low-invasive blood plasma analysis. The developed diagnostic models demonstrated high potential for clinical use and further research in this direction. |
| Databáze: | OpenAIRE |
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