The Alleviating Effect of Herniarin Against Ionizing Radiation-Induced Genotoxicity and Cytotoxicity in Human Peripheral Blood Lymphocytes
| Autor: | Sean L. Kitson, Luigi Mansi, Elham Al Fares, Mikaeil Molazadeh, Tamar Sanikidze, Sophio Kalmakhelidze, David Topuria |
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| Rok vydání: | 2022 |
| Předmět: |
Proliferation index
Radiation-Protective Agents medicine.disease_cause chemistry.chemical_compound Radiation Ionizing Herniarin medicine Humans Radiology Nuclear Medicine and imaging Lymphocytes Umbelliferones Cytotoxicity Cells Cultured Pharmacology chemistry.chemical_classification Reactive oxygen species Chemistry Coumarin Molecular biology Gamma Rays Apoptosis Micronucleus test Radiopharmaceuticals Reactive Oxygen Species Genotoxicity DNA Damage |
| Zdroj: | Current Radiopharmaceuticals. 15:141-147 |
| ISSN: | 1874-4710 |
| DOI: | 10.2174/1874471014666211012104808 |
| Popis: | Background and Objective: Herniarin is a simple coumarin that is found naturally in some plant species. In this study, we aimed to evaluate the protective effect of herniarin against ionizing radiation-induced genotoxicity and cytotoxicity in human peripheral blood lymphocytes. Methods: Herniarin was added to human lymphocytes before irradiation with a dose of 2 Gy of Xrays. The antagonistic potential of herniarin against radiation was measured by MTT [3-(4,5- dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay, micronucleus assay, flow cytometry, and reactive oxygen species (ROS) level analysis. Results: The maximum survival of lymphocytes against radiation was observed at a concentration of 50 μM of herniarin and a treatment time of 1 h. Pretreatment with 50 μM herniarin significantly decreased the micronuclei frequency, the percentage of apoptotic lymphocytes, and the ROS level in irradiated human lymphocytes. Moreover, 50 μM herniarin significantly increased the cytokinesis blocked proliferation index in irradiated lymphocytes. Conclusion: Herniarin could reduce radiation-induced cytotoxicity and genotoxicity in human lymphocytes. To complete the results of this study, it is suggested that in the future, more preclinical studies with larger samples or animal models be performed on herniarin. |
| Databáze: | OpenAIRE |
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