Disk Carbapenemase Test for the Rapid Detection of KPC-, NDM-, and Other Metallo-β-Lactamase-Producing Gram-Negative Bacilli
| Autor: | Dongeun Yong, Wonkeun Song, Hyun-Chul Kim, Seok Jeong, Kyungwon Lee, Ji Yeon Sung, Yunsop Chong |
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| Rok vydání: | 2016 |
| Předmět: |
Paper
0301 basic medicine Bacilli Imipenem 030106 microbiology Clinical Biochemistry Microbial Sensitivity Tests Biology medicine.disease_cause Rapid detection beta-Lactamases Metallo β lactamase Microbiology 03 medical and health sciences Bacterial Proteins Drug Resistance Bacterial Gram-Negative Bacteria medicine Escherichia coli Optimal treatment Biochemistry (medical) Metallo-β-lactamase General Medicine Gram negative bacilli biology.organism_classification Anti-Bacterial Agents Clinical Microbiology BROMTHYMOL BLUE Disk carbapenemase test Bromthymol Blue Original Article NDM-type carbapenemase KPC-type carbapenemase medicine.drug |
| Zdroj: | Annals of Laboratory Medicine |
| ISSN: | 2234-3814 2234-3806 |
| DOI: | 10.3343/alm.2016.36.5.434 |
| Popis: | Background Rapid detection of carbapenemase-producing gram-negative bacilli (GNB) is required for optimal treatment of infected patients. We developed and assessed a new disk carbapenemase test (DCT). Methods Paper disks containing 0.3 mg of imipenem and bromothymol blue indicator were developed, and the performance of the DCT were evaluated by using 742 strains of GNB with or without carbapenemases. Results The paper disks were simple to prepare, and the dried disks were stable at -20°C and at 4°C. The DCT detected 212 of 215 strains (98.6% sensitivity with 95% confidence interval [CI] 96.0-99.5%) of GNB with known class A (KPC and Sme) and class B (NDM, IMP, VIM, and SIM) carbapenemases within 60 min, but failed to detect GES-5 carbapenemase. The DCT also detected all two Escherichia coli isolates with OXA-48, but failed to detect GNB with OXA-232, and other OXA carbapenemases. The DCT showed 100% specificity (95% CI, 99.2-100%) in the test of 448 imipenem-nonsusceptible, but carbapenemase genes not tested, clinical isolates of GNB. Conclusions The DCT is simple and can be easily performed, even in small laboratories, for the rapid detection of GNB with KPC, NDM and the majority of IMP, VIM, and SIM carbapenemases. |
| Databáze: | OpenAIRE |
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