Maturing heart muscle cells: Mechanisms and transcriptomic insights
| Autor: | Kenneth R. Boheler, Elaine Zhelan Chen, Sean Murphy, Chulan Kwon, Leslie Tung |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell Maturation metrics Biology Article Heart development Transcriptome 03 medical and health sciences 0302 clinical medicine Pluripotent stem cells health services administration Transcriptional regulation medicine Myocyte Humans Myocytes Cardiac Induced pluripotent stem cell Cardiomyocyte maturation health care economics and organizations Regulation of gene expression Tissue Engineering Cell Differentiation Cell Biology Cell function Cell biology 030104 developmental biology medicine.anatomical_structure 030217 neurology & neurosurgery Single-cell transcriptomics Developmental Biology |
| Zdroj: | Seminars in cell & developmental biology |
| ISSN: | 1096-3634 |
| Popis: | Cardiomyocyte (CM) maturation is the transformation of differentiated fetal CMs into adult CMs that involves changes in morphology, cell function and metabolism, and the transcriptome. This process is, however, incomplete and ultimately arrested in pluripotent stem cell-derived CMs (PSC-CMs) in culture, which hinders their broad biomedical application. For this reason, enormous efforts are currently being made with the goal of generating mature PSC-CMs. In this review, we summarize key aspects of maturation observed in native CMs and discuss recent findings on the factors and mechanisms that regulate the process. Particular emphasis is put on transcriptional regulation and single-cell RNA-sequencing analysis that has emerged as a key tool to study time-series gene regulation and to determine the maturation state. We then discuss different biomimetic strategies to enhance PSC-CM maturation and discuss their effects at the single cell transcriptomic and functional levels. |
| Databáze: | OpenAIRE |
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