Isolation and characterization of wetland VSW-3, a novel lytic cold-active bacteriophage of Pseudomonas fluorescens
| Autor: | Lianbing Lin, Anxiu Kuang, Kunhao Qin, Chunjing Zhang, Shengting Zhang, Qi Zhang, Xiuling Ji, Yafang Ding, Yunlin Wei |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology Immunology Pseudomonas fluorescens Wetland Applied Microbiology and Biotechnology Microbiology Bacteriophage 03 medical and health sciences Milk products Genetics Environmental improvement Molecular Biology geography geography.geographical_feature_category biology General Medicine biology.organism_classification Isolation (microbiology) Podoviridae Cold Temperature 030104 developmental biology Lytic cycle Wetlands Pseudomonas Phages |
| Zdroj: | Canadian journal of microbiology. 63(2) |
| ISSN: | 1480-3275 |
| Popis: | Wetlands are often called the “kidneys of the Earth” and contribute substantially to environmental improvement. Pseudomonas fluorescens is a major contaminant of milk products and causes the spoilage of refrigerated foods and fresh poultry. In this study, we isolated and characterized a lytic cold-active bacteriophage named VSW-3 together with P. fluorescens SW-3 cells from the Napahai wetland in China. Electron microscopy showed that VSW-3 had an icosahedral head (56 nm) and a tapering tail (20 nm × 12 nm) and a genome size of approximate 40 kb. On the basis of the top-scoring hits in the BLASTP analysis, VSW-3 showed a high degree of module similarity to the Pseudomonas phages Andromeda and Bf7. The latent and burst periods were 45 and 20 min, respectively, with an average burst size of 90 phage particles per infected cell. The pH and thermal stability of VSW-3 were also explored. The optimal pH was found to be 7.0 and the activity decreased rapidly when the temperature exceeded 60 °C. VSW-3 is a cold-active bacteriophage, hence, it is important to research its ability to prevent product contamination caused by P. fluorescens and to characterize its relationship with its host P. fluorescens in the future. |
| Databáze: | OpenAIRE |
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