Evidence for a role of nerve injury in painful intervertebral disc degeneration: A cross-sectional proteomic analysis of human cerebrospinal fluid
| Autor: | George L. Wilcox, Troy Reihsen, Sylvie Laboissiere, Kathleen Anderson, Tony K.Y. Lim, Laura S. Stone, David S. Beebe, Kumar G. Belani, Manuel R. Pinto, Lois J. Kehl, Marcos Di Falco, Pawan Hari |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Proteomics Pathology medicine.medical_specialty Proteome Inflammation Intervertebral Disc Degeneration Asymptomatic Gastroenterology Article 03 medical and health sciences Young Adult 0302 clinical medicine Cerebrospinal fluid Hemopexin Peripheral Nerve Injuries Internal medicine medicine Humans Cystatin C Aged biology business.industry Intervertebral disc Nerve injury Middle Aged Low back pain body regions 030104 developmental biology Anesthesiology and Pain Medicine medicine.anatomical_structure Cross-Sectional Studies Neurology biology.protein Female Neurology (clinical) medicine.symptom business Low Back Pain 030217 neurology & neurosurgery Biomarkers |
| Popis: | Intervertebral disc degeneration (DD) is a cause of low back pain (LBP) in some individuals. However, although >30% of adults have DD, LBP only develops in a subset of individuals. To gain insight into the mechanisms underlying nonpainful versus painful DD, human cerebrospinal fluid (CSF) was examined using differential expression shotgun proteomic techniques comparing healthy control participants, subjects with nonpainful DD, and patients with painful DD scheduled for spinal fusion surgery. Eighty-eight proteins were detected, 27 of which were differentially expressed. Proteins associated with DD tended to be related to inflammation (eg, cystatin C) regardless of pain status. In contrast, most differentially expressed proteins in DD-associated chronic LBP patients were linked to nerve injury (eg, hemopexin). Cystatin C and hemopexin were selected for further examination using enzyme-linked immunosorbent assay in a larger cohort. While cystatin C correlated with DD severity but not pain or disability, hemopexin correlated with pain intensity, physical disability, and DD severity. This study shows that CSF can be used to study mechanisms underlying painful DD in humans, and suggests that while painful DD is associated with nerve injury, inflammation itself is not sufficient to develop LBP. Perspective CSF was examined for differential protein expression in healthy control participants, pain-free adults with asymptomatic intervertebral DD, and LBP patients with painful intervertebral DD. While DD was related to inflammation regardless of pain status, painful degeneration was associated with markers linked to nerve injury. |
| Databáze: | OpenAIRE |
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