Impact of Holder pasteurization on immunological properties of human breast milk over the first year of lactation
| Autor: | Ana Hernández, Paula Arbildi, Lilian Carroscia, Mara Castro, Laura Fazio, Cecilia Sóñora, Arturo Puyol, Claudio Rodríguez-Camejo, Emilia Villamil |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Time Factors Pasteurization Biology law.invention 03 medical and health sciences chemistry.chemical_compound Young Adult fluids and secretions 0302 clinical medicine Immune system law 030225 pediatrics Lactation medicine Humans Food science Milk Banks Milk Human Breast Milk Expression NF-kappa B food and beverages Epithelial Cells Raw milk medicine.anatomical_structure chemistry Pediatrics Perinatology and Child Health biology.protein Colostrum Female Antibody Lysozyme HT29 Cells 030217 neurology & neurosurgery |
| Zdroj: | Pediatric research. 87(1) |
| ISSN: | 1530-0447 |
| Popis: | Background The timing of milk donations to human milk banks ranges from a few days to more than 1 year after delivery, and the Holder method is used for pasteurization. We evaluated the effect of temporal variation and thermal treatment on the immunological properties of milk. Methods We analyzed 73 milk samples, raw and after pasteurization, donated at different lactation stages. We studied antibodies, lysozyme, cytokines, soluble receptors, and factors with impact on barrier function. We also evaluated in vitro the capacity of milk to modulate nuclear factor-κB (NF-κB) signaling in an HT-29 epithelial cell line stimulated with tumor necrosis factor-α (TNF-α). Results With few exceptions, immune components exhibited their highest levels in colostrum, and were stable in the various stages of mature milk. Pasteurization altered the immunological composition of milk, and very drastically for some components. Raw milk of the first year reduced NF-κB activation in HT-29 cells treated with TNF-α to approximately the same extent, and Holder pasteurization significantly affected this capacity. Conclusions Overall, the present work reports that mature donated milk is equally valuable over the first year of lactation, but warns about drastic losses of anti-inflammatory properties during Holder pasteurization that could be critical for the health of preterm infants. |
| Databáze: | OpenAIRE |
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