Isolation and characterization of rufoxin, a novel protein exhibiting neurotoxicity from venom of the psammophiine, Rhamphiophis oxyrhynchus (Rufous beaked snake)
| Autor: | Sanjaya Kuruppu, Wayne C. Hodgson, Yajnavalka Banerjee, R. Manjunatha Kini, Natalie G. Lumsden |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
Carbachol Neurotoxins Neuromuscular Junction Boiga dendrophila Venom In Vitro Techniques complex mixtures Cellular and Molecular Neuroscience Rufous beaked snake medicine Animals Neurotoxin Structural motif Chromatography High Pressure Liquid Pharmacology Analysis of Variance Dose-Response Relationship Drug biology Colubridae Anatomy biology.organism_classification Molecular biology Neostigmine Nicotinic agonist Snake venom Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cholinesterase Inhibitors Chickens Muscle Contraction Snake Venoms medicine.drug |
| Zdroj: | Neuropharmacology. 52:1065-1070 |
| ISSN: | 0028-3908 |
| Popis: | Colubrid snake venoms potentially represent a vast source of novel biological actives and structural motifs owing to their diverse phylogeny. The present study describes the identification of rufoxin, a neurotoxin from the venom of Rhamphiophis oxyrhynchus (Rufous beaked snake) which is a member of the African colubrid lineage, the psammophiines. Rufoxin (1 mu M) displayed reversible post-synaptic neurotoxic activity as evidenced by significant inhibition of indirect twitches and responses to exogenous nicotinic agonists in the chick biventer cervicis nerve-muscle preparation. Rufoxin (0.1-1.0 mu M) also caused a rightward parallel shift of cumulative concentration-response curves to carbachol (CCh; 0.6-80 mu M) without a significant depression of the maximum response, suggestive of classical competitive antagonism at the skeletal muscle nicotinic receptor. Rufoxin lacks NH2-terminal sequence homology to previously identified snake venom toxins. This work indicates a wider distribution of neurotoxins across the advanced snake superfamily than previously described. (c) 2006 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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