Cytoplasmic lipid droplets and mitochondrial distribution in equine oocytes: Implications on oocyte maturation, fertilization and developmental competence after ICSI
| Autor: | Ghylène Goudet, Sylvie Mugnier, Giovanni Michele Lacalandra, T. De Santis, A.I. Iorga, R. Matarrese, B. Ambruosi, Maria Elena Dell'Aquila |
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| Přispěvatelé: | Department of Animal Production, Università degli studi di Bari Aldo Moro (UNIBA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Cytoplasm
medicine.medical_treatment Embryonic Development intracytoplasmic sperm injection Biology Intracytoplasmic sperm injection Andrology 03 medical and health sciences Polar body 0302 clinical medicine Human fertilization Food Animals Meiosis intracytoplasmic sperm injection isci Lipid droplet Botany medicine Animals Horses Sperm Injections Intracytoplasmic Small Animals oocyte 030304 developmental biology equine cytoplasmic fluid droplets 0303 health sciences 030219 obstetrics & reproductive medicine mitochondrial distribution [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Embryogenesis Embryo Oocyte Lipids Mitochondria medicine.anatomical_structure Fertilization Oocytes Animal Science and Zoology |
| Zdroj: | Theriogenology 16. International Congress on Animal Reproduction 16. International Congress on Animal Reproduction, Jul 2008, Budapest, Hungary. ⟨10.1016/j.theriogenology.2008.12.002⟩ |
| Popis: | International audience; Lipid droplets (LDs) and mitochondria in the ooplasm are essential for energy production required for maturation, fertilization and embryo development. This Study investigates the correlations between cytoplasmic LDs polar aggregation and: (1) nuclear maturation (Experiment 1): (2) mitochondrial (nit) distribution pattern and localization (Experiment 2): (3) fertilization and embryonic development after intracytoplasmic sperm injection (ICSI; Experiment 3) in equine oocytes recovered from slaughtered mares and matured in vitro. Morphologically normal oocytes were selected after culture and categorized as having polar (P) aggregation or uniform (U) distribution of LDs. In Experiment 1. the maturation rate was significantly higher in P compared with U oocytes (69%, 40/58 vs. 32%, 13/41: P < 0.001). In Experiment 2. it was observed that P and U oocytes showed heterogeneous mt distribution at comparable rates (68%, 25/37 vs. 50%, 2/4 for P and U respectively; NS). Moreover, only in 8/25 (32%) of P oocytes. LDs overlapped with nit aggregates in the area containing meiotic spindle. In Experiment 3. normal fertilization (51%, 19/37 vs. 60%. 6/10. for P and U) and cleavage rates (83%, 20/24 vs. 67%, 4/6, for P and U) did not differ between groups, also in oocytes with LDs located nearby the polar body. Overall, P aggregation of LDs was related to cumulus of expansion at collection. In conclusion, in equine matured oocytes. P aggregation of LDs is related with cumulus expansion and nuclear maturation. However, it is not related with heterogeneous mt distribution and cannot be considered a predictive indicator for normal fertilization and embryo. |
| Databáze: | OpenAIRE |
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