Comparison of sensitivity towards photodynamic therapy of cutaneous resident and infiltrating cell types in vitro

Autor: Angelika Rück, Wolfram Sterry, Roland Kaufmann, Janice Naumann, Wolf-H. Boehncke
Rok vydání: 1996
Předmět:
Keratinocytes
Pathology
medicine.medical_specialty
Skin Neoplasms
Lymphoma
medicine.medical_treatment
Photochemotherapy
Photodynamic therapy
Keratinocytes/metabolism/*radiation effects
Dermatology
Biology
Cell Line
Mice
chemistry.chemical_compound
Lymphocytes
Tumor-Infiltrating
In vivo
Skin Neoplasms/drug therapy
Tumor Cells
Cultured
medicine
Animals
Humans
Lymphocytes
Tumor-Infiltrating/metabolism/*radiation effects
Photosensitizer
Lymphoma/drug therapy
Mycosis fungoides
Fibroblasts/metabolism/*radiation effects
Photosensitizing Agents
Methylene Blue/pharmacokinetics/therapeutic use
Cell growth
Aminolevulinic Acid
Fibroblasts
medicine.disease
Photosensitizing Agents/pharmacokinetics/therapeutic use
Aminolevulinic Acid/pharmacokinetics/therapeutic use
Methylene Blue
Hematoporphyrins
HaCaT
medicine.anatomical_structure
chemistry
Cancer research
Surgery
Keratinocyte
Methylene blue
Zdroj: Lasers in Surgery and Medicine, Vol. 19, No 4 (1996) pp. 451-457
ISSN: 1096-9101
0196-8092
DOI: 10.1002/(sici)1096-9101(1996)19:4<451::aid-lsm11>3.0.co;2-5
Popis: BACKGROUND AND OBJECTIVE: Photodynamic therapy (PDT) combines photosensitizers absorbing light in the visible spectral region and irradiation with light of corresponding wavelengths. We analysed the sensitivity of cell lines established from resident cutaneous cells and from transformed lymphocytes towards PDT. STUDY DESIGN/MATERIALS AND METHODS: PDT was performed employing either 630 or 662 nm light or polychromatic red light (600-700 nm) and photosensitizers Photosan-3, delta-aminolevulinic acid, or methylene blue. Proliferation measured by 3H-TdR uptake was determined in human immortalized keratinocytes (HaCaT) and mouse fibroblasts (NIH/3T3) in comparison to human transformed T-(HuT78) and B-lymphocytes (RA1). Additionally, uptake of the photosensitizers was estimated employing video-intensified fluorescence-microscopy (VIFM). RESULTS: Depending on the photosensitizer tested HaCaT and NIH/3T3 exhibited an ED50 up to 10-fold as high as the lymphocytic lines. Polychromatic red light was at least as effective at inducing photodynamic reactions as 630 or 662 nm light. VIFM revealed a positive correlation between sensitivity of a given cell type towards PDT and uptake of the photosensitizers. The differential uptake observed in vitro was confirmed in vivo: A photosensitizer applied topically on a lesion of a patient with mycosis fungoides was found to accumulate preferentially in the lymphocytic infiltrate. CONCLUSION: Selective topical polychromatic PDT seems to be a feasible goal for the treatment of cutaneous lymphomas.
Databáze: OpenAIRE
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