Preparative enrichment of human tissue cells capable to change a site of growth in vitro or in vivo - Recent developments
| Autor: | Johann Bauer, Hari H. P. Cohly, Jayashree Sahana, Daniela Grimm |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell type Integrin Cell Biochemistry 03 medical and health sciences 0302 clinical medicine Cell Movement In vivo medicine Humans Cells Cultured biology Weightlessness General Medicine In vitro Cell biology Fibronectin 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Organ Specificity 030220 oncology & carcinogenesis Cancer cell biology.protein RNA Function (biology) Biotechnology |
| Zdroj: | Bauer, J, Cohly, H H P, Sahana, J & Grimm, D 2018, ' Preparative enrichment of human tissue cells capable to change a site of growth in vitro or in vivo-Recent developments ', Preparative biochemistry & biotechnology, vol. 48, no. 10, pp. 954-960 . https://doi.org/10.1080/10826068.2018.1525567 |
| DOI: | 10.1080/10826068.2018.1525567 |
| Popis: | Human cells are heterogeneous in regard to their biochemical features and functions. Detailed knowledge about each single cell type is important to understand the whole organism. In order to get a deeper insight in the concert of life, it has to be considered that cell populations such as thyroid cells, epithelial breast cells, endothelial cells, or chondrocytes are heterogeneous in regard to function, RNA expression patterns and protein content. This is true for normal cells and even more relevant for cancer cells. A number of sophisticated methods were developed to enrich cohorts of cells generally belonging to a defined type but outstanding by distinct characteristics, which can be detected by microscopic, proteomic or genomic methods. There is a great interest to investigate human cells, which are able to change their site of growth within the human body leaving an original site, migrating through vessels and reentering another site. In this review experiments are summarized showing that the application of microgravity-exposure of human cells and cell electrophoresis enable a characterization of cells, which leave a site of growth to enter another one. Biochemical features of separated subpopulations are described and their usefulness for deeper investigation is highlighted. |
| Databáze: | OpenAIRE |
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