Comparative Mitogenic Effects of Herpes Simplex Virus and Mycoplasma on Murine Lymphocytes
| Autor: | David T. Kingsbury, Diane Y. Mochizuki, James D. Watson |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
viruses
Mitosis Spleen Stimulation Biology medicine.disease_cause Microbiology Mice chemistry.chemical_compound Mycoplasma Virology medicine Animals Simplexvirus Lymphocytes DNA synthesis Chloramphenicol DNA Transfection Herpes simplex virus medicine.anatomical_structure chemistry Antibody Formation Thymidine medicine.drug |
| Zdroj: | Journal of General Virology. 54:185-190 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-54-1-185 |
| Popis: | Summary Previous work indicated that herpes simplex virus type 1 (HSV-1) is a mitogen for mouse spleen cultures, as monitored by uptake of 3H-thymidine. We observed variable responses of mouse spleen cultures to different viral preparations. The variable responses, which did not follow normal dose—response relationships, were not due to HSV-1 strain differences, altered response kinetics or the presence or absence of defective viral particles, but to mycoplasma contamination of viral stocks. Mycoplasma-free (MF) HSV-1 stocks were prepared by transfection of MF NHF cells with HSV-1 DNA. MF HSV-1 infection of spleen cultures resulted in a five- to sixfold stimulation of DNA synthesis and stimulation of a polyclonal antibody response. Heat treatment (56 °C for 1 h) and antibiotics were used to distinguish mycoplasma and HSV-1-induced spleen culture mitogenic responses. The mycoplasma-induced mitogenic activity was found to be heat labile and sensitive to gentamicin and chloramphenicol. In contrast, the HSV-1-induced response was not affected by gentamicin or chloramphenicol and heat treatment resulted in only a 50% loss of activity. |
| Databáze: | OpenAIRE |
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