CDKN1C (P57): one of the determinants of human endometrial stromal cell decidualization
Autor: | Hui Yang, Dan Hu, Jianyuan Song, Kun Qian, Xuejiao Sun, Hanwang Zhang, Liu Jiang, Lan Wang, Licheng Ji, Linli Hu, Jackson Ferdinand Masau, Shuxia Ma |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Endometrium 03 medical and health sciences Cyclin D1 Decidua Humans Cyclin-Dependent Kinase Inhibitor p57 Transcription factor Cyclin-Dependent Kinase Inhibitor p15 Endometrial Stromal Cell Cyclin-dependent kinase 1 biology urogenital system Kinase Cell Cycle Cyclin-Dependent Kinase 2 Cyclin-dependent kinase 2 Decidualization Cell Differentiation Cell Biology General Medicine Cell cycle Cell biology Genes cdc 030104 developmental biology Reproductive Medicine embryonic structures biology.protein Female RNA Interference Stromal Cells biological phenomena cell phenomena and immunity Signal Transduction |
Zdroj: | Biology of Reproduction. 98:277-285 |
ISSN: | 1529-7268 0006-3363 |
Popis: | Decidualization is regulated by crosstalk of progesterone and the cAMP pathway. It involves extensive reprogramming of gene expression and includes a wide range of functions. To investigate how cell cycle regulatory genes drive the human endometrial stromal cell (ESC) exit cell cycle and enter differentiation, primary cultured ESC was treated with 8-Br-cAMP and MPA and cell cycle distribution was investigated by flow cytometry. High-throughput cell cycle regulatory gene expression was also studied by microarray. To validate the results of microarray chip, immunohistochemistry and semi-quantitative method of optical density were used to analyze the expression of cell cycle regulator proteins in proliferative phase of endometrium (n = 6) and early pregnancy decidua (n = 6). In addition, we selected cyclin-dependent kinase inhibitor 1c (CDKN1C, also known as P57) and cyclin-dependent kinase inhibitor 2b (CDKN2B, also known as P15) in order to study their role in the process of decidualization by the RNAi method. ESC was arrested at G0/G1 checkpoints during decidualization. Cell cycle regulatory genes P57 and P15 were upregulated, while cyclin D1 (CCND1), cyclin-dependent kinase 2 (CDK2), and cell division cycle protein 2 homolog (CDC2) were downregulated during ESC differentiation both in vitro and vivo. P57 siRNA impaired ESC decidualization and caused differentmorphological and ultrastructural changes as well as a relatively low secretion of prolactin, but P15 siRNA had no effects. We concluded that P15, CCND1, CDK2, and CDC2 may participate in ESC withdraw from the cell cycle and go into differentiation both in vitro and in vivo. P57 is one of the key determinants of ESC differentiation due to its effect on the cell cycle distribution, but its association with the decidua-specific transcription factor needs further investigation.Summary SentenceCell cycle regulatory genes P57 and P15 are upregulated, while Cyclin D1, CDK2, and CDC2 are downregulated during the ESC differentiation and P57 is one of the key regulators that modulates ESC differentiation by controlling cell cycle distribution. |
Databáze: | OpenAIRE |
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