Template-directed RNA polymerization and enhanced ribozyme catalysis inside membraneless compartments formed by coacervates
| Autor: | Erica A. Frankel, Christine D. Keating, Rebecca M. Guth-Metzler, Andrew J. Veenis, Philip C. Bevilacqua, Raghav R. Poudyal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Science Aptamer Deoxyribozyme General Physics and Astronomy 02 engineering and technology Article General Biochemistry Genetics and Molecular Biology Polymerization 03 medical and health sciences Polylysine RNA Catalytic lcsh:Science chemistry.chemical_classification Multidisciplinary Coacervate biology Chemistry Ribozyme RNA General Chemistry 021001 nanoscience & nanotechnology Quaternary Ammonium Compounds 030104 developmental biology Enzyme biology.protein Biophysics Nucleic acid Nucleic Acid Conformation lcsh:Q Polyethylenes Peptides 0210 nano-technology |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Membraneless compartments, such as complex coacervates, have been hypothesized as plausible prebiotic micro-compartments due to their ability to sequester RNA; however, their compatibility with essential RNA World chemistries is unclear. We show that such compartments can enhance key prebiotically-relevant RNA chemistries. We demonstrate that template-directed RNA polymerization is sensitive to polycation identity, with polydiallyldimethylammonium chloride (PDAC) outperforming poly(allylamine), poly(lysine), and poly(arginine) in polycation/RNA coacervates. Differences in RNA diffusion rates between PDAC/RNA and oligoarginine/RNA coacervates imply distinct biophysical environments. Template-directed RNA polymerization is relatively insensitive to Mg2+ concentration when performed in PDAC/RNA coacervates as compared to buffer, even enabling partial rescue of the reaction in the absence of magnesium. Finally, we show enhanced activities of multiple nucleic acid enzymes including two ribozymes and a deoxyribozyme, underscoring the generality of this approach, in which functional nucleic acids like aptamers and ribozymes, and in some cases key cosolutes localize within the coacervate microenvironments. Membraneless compartments have been theorized to be prebiotic micro-compartments as they spontaneously encapsulate RNA and proteins. Here, the authors report membraneless compartments can enhance RNA chemistries, affecting template directed RNA polymerization and stimulating nucleic acid enzymes. |
| Databáze: | OpenAIRE |
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