Identification of TOSO/FAIM3 as an Fc receptor for IgM
| Autor: | Hiroshi Ohno, Ji-Yang Wang, Shinji Fukuda, Hideaki Shima, Hiroyuki Takatsu, Hiromi Kubagawa, Masumi Ohmae, Koji Hase |
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| Rok vydání: | 2009 |
| Předmět: |
Molecular Sequence Data
Immunology B-Lymphocyte Subsets Fc receptor Cell Separation Receptors Fc Immunoglobulin domain Transfection Mice Antigen medicine Animals Humans Immunology and Allergy Amino Acid Sequence Receptor B cell B-Lymphocytes biology Reverse Transcriptase Polymerase Chain Reaction Membrane Proteins General Medicine Blotting Northern Flow Cytometry Fragment crystallizable region Molecular biology Mice Inbred C57BL medicine.anatomical_structure Immunoglobulin M biology.protein Apoptosis Regulatory Proteins Carrier Proteins Sequence Alignment HeLa Cells |
| Zdroj: | International Immunology. 22:149-156 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/dxp121 |
| Popis: | Fc receptors specifically bind to the Fc region of Igs to mediate the unique functions to each class of Igs. To identify a novel Fc receptor for IgM, we searched expressed sequence tag database for molecules containing Ig domains with homology to those of known Fc receptors for IgM, Fcalpha/muR and polymeric Ig receptor. As a result, we identified TOSO/Fas apoptotic inhibitory molecule 3 (FAIM3) as a possible Fc receptor for IgM. HeLa cells transfected with a TOSO/FAIM3-expression vector bound to IgM but not IgG and were able to internalize IgM-conjugated beads but not IgG-conjugated beads, suggesting that TOSO/FAIM3 is indeed a receptor for IgM (FcmuR). FcmuR protein was expressed predominantly on B-lineage cells; expression of the Fcmr transcripts was observed from the pre-B-cell stage and maintained thereafter during B-cell development. These results identify TOSO/FAIM3 as a receptor for IgM and suggest that FcmuR may serve as an uptake receptor for IgM-opsonized antigens by B cells. |
| Databáze: | OpenAIRE |
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