Development of alginate dialdehyde-gelatin based bioink with methylcellulose for improving printability
Autor: | Dalia Dranseikiene, Dirk W. Schubert, Aldo R. Boccaccini, Supachai Reakasame, Stefan Schrüfer, Kai Zheng |
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Rok vydání: | 2021 |
Předmět: |
Materials science
food.ingredient Alginates Cell Survival Bioengineering Toxic potential macromolecular substances Methylcellulose Gelatin Biomaterials food medicine Cell damage Incubation Cell spreading Tissue Scaffolds technology industry and agriculture Bioprinting Hydrogels medicine.disease Chemical engineering Mechanics of Materials Cell culture Self-healing hydrogels Printing Three-Dimensional Biofabrication |
Zdroj: | Materials scienceengineering. C, Materials for biological applications. 128 |
ISSN: | 1873-0191 |
Popis: | This study used methylcellulose (MC) to improve the printability of the alginate dialdehyde-gelatin (ADA-GEL) based bioink. The printability as well as the capability to maintain shape fidelity of ADA-GEL could be enhanced by the addition of 9% (w/v) MC. Moreover, the properties of the ink crosslinked with Ca2+ and Ba2+ were investigated. The samples crosslinked with Ba2+ were more stable and stiffer than the Ca2+ crosslinked samples. However, both Ca2+ and Ba2+ crosslinked samples exhibited a similar trend of MC release during incubation under cell culture conditions. The toxicity test indicated that both samples (crosslinked with Ca2+ and Ba2+) exhibited no toxic potential. The fabrication of cell-laden constructs using the developed bioinks was evaluated. The viability of ST2 cells in Ba2+ crosslinked samples increased while for Ca2+ crosslinked samples, a decreased viability was observed over the incubation time. After 21 days, cell spreading in the hydrogels crosslinked with Ba2+ occurred. However, a certain degree of cell damage was observed after incorporating the cells in the high viscosity bioink. |
Databáze: | OpenAIRE |
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