On the Origin of Striatal Cholecystokinin Release: Studies with In Vivo Microdialysis
| Autor: | Mario Herrera-Marschitz, Tomas Hökfelt, J. Javier Meana, Ernst Brodin, P. Morino, Zhi-Bing You, U. Ungersfedt, Michel Goiny, Rodolfo Silveira |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Microdialysis Dopamine Radioimmunoassay Glutamic Acid Biology digestive system Biochemistry Sincalide Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Glutamates Internal medicine medicine Animals Oxidopamine Neurotransmitter Medial forebrain bundle Chromatography High Pressure Liquid Cholecystokinin Afferent Pathways Aspartic Acid digestive oral and skin physiology Homovanillic acid Glutamate receptor Homovanillic Acid Corpus Striatum Rats Endocrinology Monoamine neurotransmitter nervous system chemistry Potassium 3 4-Dihydroxyphenylacetic Acid Calcium hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Journal of Neurochemistry. 62:76-85 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1994.62010076.x |
| Popis: | In the present study, extracellular levels of the neuropeptide cholecystokinin (CCK), of the monoamine dopamine and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), and of the excitatory amino acids glutamate and aspartate were simultaneously monitored by microdialysis in the neostriatum of halothane-anesthetized rats under basal and K(+)-depolarizing conditions. Extracellular CCK and dopamine levels, but not glutamate and aspartate levels, were decreased by perfusion with a Ca(2+)-free medium, under both basal and K(+)-depolarizing conditions. HPLC revealed that the majority of the CCK-like immunoreactivity in the perfusates coeluted with CCK octapeptide. Striatal extracellular CCK levels were decreased by decortication plus callosotomy, with a parallel decrease in glutamate levels. Striatal extracellular levels of dopamine, DOPAC, and HVA were significantly decreased in animals treated previously with a unilateral 6-hydroxydopamine injection into the medial forebrain bundle. In these animals, however, the effect of decortication plus callosotomy on CCK and glutamate levels was not further augmented. Thus, this study supports the hypothesis of a neuronal origin of extracellular CCK and dopamine monitored with microdialysis in the striatum of the rat, and also supports the idea of a partly contralateral origin of corticostriatal CCK and glutamate inputs. |
| Databáze: | OpenAIRE |
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