Idiotype-pulsed dendritic cell vaccination for B-cell lymphoma: clinical and immune responses in 35 patients
| Autor: | Adrienne van Beckhoven, Thomas A. Davis, Edgar G. Engleman, Behnaz Taidi, Craig Okada, Claudia Benike, Debra K. Czerwinski, Ronald Levy, Tina Marie Liles, Ranjani Rajapaksa, John M. Timmerman, Frank J. Hsu, Clemens B. Caspar, Zheng Mei Hao |
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| Rok vydání: | 2002 |
| Předmět: |
Idiotype
Adult Male Lymphoma B-Cell medicine.medical_treatment T-Lymphocytes Immunology Follicular lymphoma Biochemistry Cancer Vaccines Immunotherapy Adoptive Immune system Immunoglobulin Idiotypes medicine Humans B-cell lymphoma Aged biology business.industry Cell Biology Hematology Immunotherapy Dendritic Cells Middle Aged medicine.disease Antibodies Anti-Idiotypic Vaccination Treatment Outcome Tumor progression Antibody Formation Hemocyanins biology.protein Antibody business Follow-Up Studies |
| Zdroj: | Blood. 99(5) |
| ISSN: | 0006-4971 |
| Popis: | Tumor-specific clonal immunoglobulin expressed by B-cell lymphomas (idiotype [Id]) can serve as a target for active immunotherapy. We have previously described the vaccination of 4 patients with follicular lymphoma using dendritic cells (DCs) pulsed with tumor-derived Id protein and now report on 35 patients treated using this approach. Among 10 initial patients with measurable lymphoma, 8 mounted T-cell proliferative anti-Id responses, and 4 had clinical responses—2 complete responses (CRs) (progression-free [PF] for 44 and 57 months after vaccination), 1 partial response (PR) (PF for 12 months), and 1 molecular response (PF for 75+ months). Subsequently, 25 additional patients were vaccinated after first chemotherapy, and 15 of 23 (65%) who completed the vaccination schedule mounted T-cell or humoral anti-Id responses. Induction of high-titer immunoglobulin G anti-Id antibodies required coupling of Id to the immunogenic carrier protein keyhole limpet hemocyanin (Id-KLH). These antibodies could bind to and induce tyrosine phosphorylation in autologous tumor cells. Among 18 patients with residual tumor at the time of vaccination, 4 (22%) had tumor regression, and 16 of 23 patients (70%) remain without tumor progression at a median of 43 months after chemotherapy. Six patients with disease progression after primary DC vaccination received booster injections of Id-KLH protein, and tumor regression was observed in 3 of them (2 CRs and 1 PR). We conclude that Id-pulsed DC vaccination can induce T-cell and humoral anti-Id immune responses and durable tumor regression. Subsequent boosting with Id-KLH can lead to tumor regression despite apparent resistance to the primary DC vaccine. |
| Databáze: | OpenAIRE |
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