Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units
| Autor: | Kriton S. Filos, Iris Spiliopoulou, Fotini Fligou, Eleanna Drougka, Vasiliki Dodou, Evangelos D. Anastassiou, Markos Marangos, Efthimia Petinaki, Fevronia Kolonitsiou, Matthaios Papadimitriou-Olivgeris |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis daptomycin critically ill patients vancomycin lcsh:Medicine MRSA Toxicology medicine.disease_cause Group A Group B Hospitals University chemistry.chemical_compound Enterotoxins Leukocidins carriage toxic shock syndrome toxin (TSST-1) Panton–Valentine leukocidin (PVL) MLST linezolid Cross Infection Superantigens Greece Toxic shock syndrome toxin Staphylococcal Infections Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Intensive Care Units Staphylococcus aureus Vancomycin medicine.drug Health Personnel 030106 microbiology Bacterial Toxins Exotoxins Microbial Sensitivity Tests Biology Article Microbiology 03 medical and health sciences Bacterial Proteins Intensive care medicine Humans Penicillin-Binding Proteins lcsh:R biochemical phenomena metabolism and nutrition bacterial infections and mycoses chemistry Linezolid Multilocus sequence typing bacteria Methicillin Resistance Multilocus Sequence Typing |
| Zdroj: | Toxins Toxins, Vol 9, Iss 9, p 270 (2017) Toxins; Volume 9; Issue 9; Pages: 270 |
| ISSN: | 2072-6651 |
| Popis: | Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton–Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton–Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR. Clones were identified in all methicillin-resistant S. aureus (MRSA) and toxin-positive methicillin-susceptible strains (MSSA) by pulsed-field gel electrophoresis (PFGE), agr groups, and multi locus sequencing typing (MLST). Group A included 90 S. aureus isolates, whereas Group B 53. PVL was more frequently found among MRSA vs. MSSA (p < 0.001) and in strains of Group B as compared to Group A (p < 0.001), consistent with the spread of ST80-IV. Higher incidence of tst gene carriage was identified among MSSA vs. MRSA (P 0.005) belonging mainly to ST30, and Group A vs. Group B (P 0.002). The wide dissemination of ST80-IV mainly in the community is responsible for a high percentage of PVL-positive MRSA, while silent spread of tst-positive S. aureus clones among ICU patients and personnel poses a threat of hospital transmission and possible severe infections. |
| Databáze: | OpenAIRE |
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