Stimulatory effect of glucose 6-phosphate on the non-mitochondrial Ca2+ uptake in permeabilized hepatocytes and Ca2+ release by inositol trisphosphate
| Autor: | Mario Comporti, Andrea Romani, Angelo Benedetti, Rosella Fulceri |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
medicine.medical_specialty Cell Membrane Permeability Inositol Phosphates Biological Transport Active Stimulation Inositol 1 4 5-Trisphosphate Biology Endoplasmic Reticulum chemistry.chemical_compound Internal medicine medicine Animals Vanadate Inositol Molecular Biology Glucosephosphates Vanadium Inositol trisphosphate Cell Biology Metabolism Saponins (Permeabilized hepatocyte) Calcium ion uptake Cell Compartmentation Rats Endocrinology medicine.anatomical_structure Glucose 6-phosphate Liver chemistry Biochemistry Hepatocyte Glucose-6-Phosphatase Microsome Calcium Sugar Phosphates |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 928:282-286 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(87)90187-x |
| Popis: | The relationships between Ca2+ transport and glucose-6-phosphatase activity, previously studied in isolated liver microsomes, were investigated in permeabilized hepatocytes in the presence of mitochondrial inhibitors. It was found that the addition of glucose 6-phosphate to the cells markedly stimulates the MgATP-dependent Ca2+ uptake. A progressive increase in the stimulation of Ca2+ uptake was seen with increasing amounts of glucose 6-phosphate up to 5 mM concentrations. Vanadate, when added in adequate concentrations (20–40 μM) to the hepatocytes inhibits both the glucose-6-phosphatase activity and the stimulation of Ca2+ uptake by glucose 6-phosphate, while not affecting the MgATP-dependent Ca2+ uptake. The addition of inositol 1,4,5-trisphosphate to permeabilized hepatocytes in which Ca2+ had been accumulated in the presence of MgATP and glucose 6-phosphate, results in a rapid release of Ca2+. |
| Databáze: | OpenAIRE |
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