Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium
| Autor: | Susanne M. A. van der Pol, Helga E. de Vries, Christine D. Dijkstra, Jacob van den Born, Sarah Floris |
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| Přispěvatelé: | Groningen Kidney Center (GKC), Groningen Institute for Organ Transplantation (GIOT), Psychiatry, Molecular cell biology and Immunology, ACS - Microcirculation, Amsterdam Neuroscience - Neuroinfection & -inflammation, Amsterdam Neuroscience - Neurovascular Disorders, CCA - Cancer biology and immunology |
| Rok vydání: | 2003 |
| Předmět: |
Male
Protein Structure Secondary/drug effects Wistar Leukocyte/drug effects Heparin/pharmacology Monocytes Protein Structure Secondary Syndecan 1 Sulfation Signal Transduction/drug effects Cell Adhesion/drug effects Syndecan-2 Encephalomyelitis Heparan Sulfate Proteoglycans/metabolism Vascular/drug effects Cerebral Cortex Membrane Glycoproteins biology Inbred Lew Sulfates Chemotaxis Membrane Glycoproteins/metabolism General Medicine Adhesion Heparin Cell biology Extracellular Matrix Chemotaxis Leukocyte medicine.anatomical_structure Neurology Encephalitis Proteoglycans Chemotaxis Leukocyte/drug effects medicine.drug Extracellular Matrix/drug effects Signal Transduction Endothelium Vascular/drug effects Protein Structure Encephalomyelitis Autoimmune Experimental Endothelium Encephalitis/drug therapy Pathology and Forensic Medicine Cellular and Molecular Neuroscience Experimental Monocytes/drug effects Sulfates/antagonists & inhibitors GTP-Binding Proteins medicine Cell Adhesion Animals GTP-Binding Proteins/antagonists & inhibitors Rats Wistar Cerebral Cortex/blood supply Animal Monocyte Proteoglycans/metabolism Rats carbohydrates (lipids) Disease Models Animal Proteoglycan Secondary/drug effects Rats Inbred Lew Immunology Disease Models biology.protein Neurology (clinical) Endothelium Vascular Heparan Sulfate Proteoglycans Autoimmune |
| Zdroj: | Floris, S, van den Born, J, van der Pol, S M A, Dijkstra, C D & De Vries, H E 2003, ' Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium ', Journal of Neuropathology and Experimental Neurology, vol. 62, no. 7, pp. 780-90 . Journal of neuropathology and experimental neurology, 62(7), 780-790. Oxford University Press Journal of Neuropathology and Experimental Neurology, 62(7), 780-90. Lippincott Williams and Wilkins |
| ISSN: | 0022-3069 |
| Popis: | Heparan sulfate proteoglycans (HSPGs) are known to participate in a wide range of biological events, including cellular trafficking. In this study we report that in situ cerebral blood vessels highly express HSPGs. Of the syndecan family, syndecan-2 is highly expressed on virtually all brain vessels and syndecan-1 and -3 are only present on larger blood vessels. These endothelial HSPGs have a functional role in monocyte diapedesis across brain endothelium, as assessed in our in vitro adhesion and migration assays. Our data indicate that heparin prevents monocyte adhesion to brain endothelium by interacting solely with the monocyte. Transendothelial migration of monocytes can be prevented by preincubation of brain endothelium with heparin by enzymatic removal of heparan sulphate side chains or by inhibition of cellular sulfation. Blocking of G-protein-dependent signaling in the monocytes prevented monocyte adhesion and migration to similar extent, suggesting that G-dependent signaling may be involved in HSPG-mediated monocyte adhesion and transendothelial migration. Our data demonstrate that brain endothelial HSPGs have a modulatory role in the transendothelial migration of monocytes in a direct and indirect fashion and may therefore contribute to the formation of neuroinflammatory lesions. |
| Databáze: | OpenAIRE |
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