Two Types of Death of Poliovirus-Infected Cells: Caspase Involvement in the Apoptosis but Not Cytopathic Effect
| Autor: | L.I. Romanova, Elena A. Tolskaya, Marina S. Kolesnikova, E. A. Smirnova, N. T. Raikhlin, Denise Egger, Kurt Bienz, Vadim I. Agol, George A. Belov |
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| Rok vydání: | 1998 |
| Předmět: |
Proteases
Programmed cell death viruses Apoptosis Cysteine Proteinase Inhibitors Amino Acid Chloromethyl Ketones 03 medical and health sciences Prophase Virology medicine Humans Guanidine Caspase 030304 developmental biology Cytopathic effect Cell Nucleus 0303 health sciences Cell Death biology 030306 microbiology Tosylphenylalanyl Chloromethyl Ketone Caspase Inhibitors Molecular biology Poliovirus Cell nucleus medicine.anatomical_structure Viral replication Caspases biology.protein HeLa Cells |
| Zdroj: | Virology. 252:343-353 |
| ISSN: | 0042-6822 |
| Popis: | The death of poliovirus-infected cells may occur in two forms: canonical cytopathic effect (CPE) (on productive infections) or apoptosis (when the viral reproduction is hindered by certain drugs or some other restrictive conditions). Morphological manifestations of the CPE and apoptosis, being distinct, share some traits (e.g., chromatin condensation and nuclear deformation). It was shown here that a permeable caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp-(OMe) fluoromethyl ketone (zVAD.fmk), prevented the development of the poliovirus-induced apoptosis on abortive infection. The apoptotic pathway could be dissected by an inhibitor of chymotrypsin-like serine proteases,N-tosyl-l-phenylalanine chloromethyl ketone (TPCK), which prevented the cleavage of DNA to oligonucleosome-sized pieces and nuclear fragmentation but did not suppress cellular shrinkage, cytoplasmic blebbing, and partial chromatin condensation. These results demonstrate that caspase activation is involved in the execution phase of the viral apoptosis and suggest that a nuclear subset of the apoptotic program is under a separate control, involving a TPCK-sensitive event. Neither zVAD.fmk nor TPCK, at the concentrations affecting the apoptotic response, exerted appreciable influence on the virus growth or cellular pathological changes on productive infection, indicating that the pathways leading to the poliovirus-evoked CPE and apoptosis are different. |
| Databáze: | OpenAIRE |
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