RhoV mediates apoptosis of RAW264.7 macrophages caused by osteoclast differentiation
| Autor: | Ruilong Song, Laiyang Cheng, Jiaming Zhang, Zongping Liu, Xuezhong Liu, Di Hu, Yan Yuan, Jianhong Gu, Jiaqiao Zhu, Qichao Wang, Qian Gao, Dong Wang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
rac1 GTP-Binding Protein
rho GTP-Binding Proteins musculoskeletal diseases Cancer Research RHOA Osteoclasts Apoptosis RAC1 Biology Biochemistry Cell Line Mice Osteoprotegerin Osteoclast Precursor cell Genetics medicine Animals RNA Messenger Molecular Biology Macrophage Colony-Stimulating Factor Macrophages RANK Ligand Cell Differentiation hemic and immune systems Cell cycle Up-Regulation Cell biology medicine.anatomical_structure Oncology RANKL Cancer research biology.protein Molecular Medicine lipids (amino acids peptides and proteins) rhoA GTP-Binding Protein |
| Zdroj: | Molecular Medicine Reports. 11:1153-1159 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2014.2817 |
| Popis: | Macrophages, a type of immune cell, are the precursors of osteoclasts, and have important roles in bone remodeling and the immune system. In the present study, the RAW264.7 cell line was used as a macrophage model in order to study the macrophage changes during osteoclastogenesis. Receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony‑stimulating factor (M‑CSF) induce the formation of osteoclasts from several precursor cells. Observation of RAW264.7 macrophage osteoclastogenesis under the induction of RANKL and M‑CSF revealed that except the few RAW264.7 macrophages that were differentiated into osteoclasts, almost all undifferentiated RAW264.7 macrophages underwent apoptosis. BRL‑3A cells have no differentiation ability, and RANKL and M‑CSF treatments did not induce BRL‑3A cell apoptosis. When osteoprotegerin (OPG) was used to completely inhibit the differentiation of RAW264.7 macrophages to osteoclasts, apoptosis did not occur amongst the RAW264.7 macrophages despite the action of RANKL and M‑CSF. Rac1, RhoA and RhoV are apoptosis‑associated genes in the Rho guanosine triphosphate (GTP)ase family. Their expression levels were detected using quantitative polymerase chain reaction (qPCR). During the process of osteoclast differentiation, the mRNA expression of RhoV was significantly upregulated, while apoptosis occurred in a large proportion of macrophages. However, when macrophage apoptosis was inhibited by OPG, RhoV expression was significantly downregulated. Conversely, Rac1 and RhoA expression did not vary in correspondence with the apoptotic rate of the RAW264.7 macrophages. In conclusion, differentiation of RAW264.7 macrophages into osteoclasts resulted in their apoptosis. OPG inhibited RAW264.7 macrophage differentiation into osteoclasts, and thereby inhibited the apoptosis of RAW264.7 macrophages. RhoV mediated the apoptosis of RAW264.7 macrophages during osteoclast differentiation. |
| Databáze: | OpenAIRE |
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