L-fucose, D-mannose, L-galactose, and their BSA conjugates stimulate macrophage migration
| Autor: | Isao Takata, M J Ricardo, Kingo Chida, Quentin N. Myrvik, Mark R. Gordon, Louis S. Kucera |
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| Rok vydání: | 1987 |
| Předmět: |
Male
Cellular immunity Immunology Serum albumin Mannose Receptors Cell Surface Biology Fucose chemistry.chemical_compound Cell Movement Immunology and Allergy Animals Bovine serum albumin Receptors Immunologic Receptor Macrophage Migration-Inhibitory Factors Cells Cultured Mannan alpha-L-Fucosidase Macrophages Lymphokine Galactose Serum Albumin Bovine Cell Biology Molecular biology Pulmonary Alveoli Biochemistry chemistry biology.protein Female Rabbits Spleen |
| Zdroj: | Journal of leukocyte biology. 41(3) |
| ISSN: | 0741-5400 |
| Popis: | The effect of selected monosaccharides on the random migration of normal adult rabbit alveolar macrophages (AM) was investigated. It was observed that 10 mM of L-fucose, L-galactose, or D-mannose stimulated AM migration 1.5-2.0 times. In addition, derivatives of L-fucose and D-mannose occupying the carbon-6 position such as L-fucosyl-lactose, D-mannose-6-phosphate, D-mannitol, and mannan enhanced the migration of AM, whereas derivatives of L-fucose and D-mannose in the carbon-1 position produced no migration enhancement. Macrophage migration enhancement activity that was produced spontaneously by spleen cell cultures from normal young rabbits was destroyed by treatment with L-fucosidase. Accordingly, the migration enhancement factor (MEF) found in spleen cell culture supernatants appeared to depend on L-fucose conjugated to some protein carrier because MEF was non-dialyzable. When normal adult AM were treated with L-fucosidase, they lost their responsiveness to migration inhibitory factor (MIF) but retained their responsiveness to MEF. We have interpreted this to mean that the MIF and MEF receptors are distinct. Synthetic MEFs were prepared by conjugating L-fucose, D-mannose, of L-galactose to bovine serum albumin (BSA). It was noted that these sugar-BSA conjugates were about 200 times more effective than the corresponding free sugars in producing migration enhancement. In addition, these sugar-BSA conjugates neutralized MIF activity in a migration inhibition test. |
| Databáze: | OpenAIRE |
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