Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain
| Autor: | Jordan Shoemaker, Marcelo E. Tolmasky, Eric Fung, Francesca de la Cruz, Jackie Zhang, Krystle Burinski, German Matias Traglia, Ashley Rivera, Maria Soledad Ramirez, Brett Farthing, Kenneth Rocha, Jennifer S. Fernandez, Kevin Van, Keizen Li Qian, Vicente Lizarraga, Kevin Doan, Alexandra J. Ferreri, Veronica Batallones, Kimberly Phan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Vibrio anguillarum Science 030106 microbiology Gene mutation medicine.disease_cause Article Frameshift mutation 03 medical and health sciences Bacterial Proteins Gene Duplication Escherichia coli Genetics medicine Gene Vibrio Homogentisate 1 2-dioxygenase Homogentisate 1 2-Dioxygenase Mutation Multidisciplinary Models Genetic biology Pigmentation Genetic Complementation Test HMGA Computational Biology DNA Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Complementation 030104 developmental biology Medicine Tyrosine DNA Intergenic Microbial genetics Genome Bacterial |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-51126-8 |
| Popis: | Vibrio anguillarum 531A, isolated from a diseased fish in the Atlantic Ocean, is a mixture composed of about 95 and 5% of highly pigmented cells (strain 531Ad) and cells with normal levels of pigmentation (strain 531Ac), respectively. Analysis of the V. anguillarum 531Ad DNA region encompassing genes involved in the tyrosine metabolism showed a 410-bp duplication within the hmgA gene that results in a frameshift and early termination of translation of the homogentisate 1,2-dioxygenase. We hypothesized that this mutation results in accumulation of homogentisate that is oxidized and polymerized to produce pyomelanin. Introduction in E. coli of recombinant clones carrying the V. anguillarum hppD (4-hydroxyphenylpyruvate-dioxygenase), and a mutated hmgA produced brown colored colonies. Complementation with a recombinant clone harboring hmgA restored the original color to the colonies confirming that in the absence of homogentisate 1,2-dioxygenase the intermediary in tyrosine catabolism homogentisate accumulates and undergoes nonenzymatic oxidation and polymerization resulting in high amounts of the brown pigment. Whole-genome sequence analysis showed that V. anguillarum 531 Ac and 531Ad differ in the hmgA gene mutation and 23 mutations, most of which locate to intergenic regions and insertion sequences. |
| Databáze: | OpenAIRE |
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