De novo transcriptome assembly of heavy metal tolerant Silene dioica
| Autor: | Vojtech Hudzieczek, Roman Hobza, Radim Cegan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 De novo transcriptome assembly Sequence assembly RNA-Seq 010603 evolutionary biology 01 natural sciences Biochemistry Transcriptome 03 medical and health sciences Data in Brief Genetics Illumina dye sequencing Silene biology Accession number (library science) food and beverages Sex chromosomes biology.organism_classification Silene dioica lcsh:Genetics 030104 developmental biology Molecular Medicine Heavy metal tolerance Biotechnology |
| Zdroj: | Genomics Data Genomics Data, Vol 11, Iss C, Pp 118-119 (2017) |
| ISSN: | 2213-5960 0000-0000 |
| Popis: | Silene dioica is a dioecious plant of the family Caryophyllaceae. In the present study, we used Illumina sequencing technology (MiSeq) to sequence, de novo assembly and annotate the transcriptomes of male and female copper tolerant S. dioica individuals. We sequenced the normalized mRNA of roots, shoots, flower buds and flowers for each sex. Raw reads of the transcriptome assembly project for S. dioica male and female individual have been deposited in NCBI's Sequence Read Archive (SRA) database with the accession number SRP094611. The Trinity and Detonate program was used to de novo assembly 92,347 transcripts for male and 94,757 transcripts for female transcriptome. The assembled transcriptome sequences for S. dioica male and female individuals can be accessed at NCBI with the following accession numbers: GFCG00000000 (male); GFCH00000000 (female). The obtained transcriptomic data will be useful for further studies focusing on copper tolerance, comparative transcriptome analysis with other Silene species and sex chromosomes evolution. |
| Databáze: | OpenAIRE |
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