DNA vaccination using coexpression of cytokine genes with a bacterial gene encoding a 60-kDa heat shock protein
| Autor: | Reinhold Schirmbeck, Annette Noll, Ingo B. Autenrieth, Jörg Reimann, Mathias W. Hornef |
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| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Interleukin 2 Cellular immunity Yersinia Infections T-Lymphocytes Immunology Genetic Vectors chemical and pharmacologic phenomena Biology Lymphocyte Activation DNA vaccination Microbiology Interferon-gamma Mice Immune system Heat shock protein medicine Vaccines DNA Immunology and Allergy Animals Interleukin 4 Yersinia enterocolitica Mice Inbred BALB C Vaccination General Medicine Chaperonin 60 Antibodies Bacterial Mice Inbred C57BL Humoral immunity Bacterial Vaccines Cytokines Interleukin-2 HSP60 Female Interleukin-4 Spleen medicine.drug Plasmids |
| Zdroj: | Medical microbiology and immunology. 189(2) |
| ISSN: | 0300-8584 |
| Popis: | Coexpression of cytokine genes together with antigen-encoding genes in DNA vaccination vectors can increase humoral and cellular immune responses and may steer them in a Th1 or Th2 direction. In this study, the modulatory effect of interleukin (IL)-2, IL-4, and interferon (IFN)-gamma coexpressed with the 60-kDa heat shock protein (Hsp60) of Yersinia enterocolitica O:8 (Y-Hsp60) was studied. DNA vaccination with gamma-hsp60 evoked specific humoral and cellular immune responses as well as reduction of the splenic bacterial load upon challenge with Y. enterocolitica in a mouse infection model. Coexpression of IL-2 or IFN-gamma enhanced Y. enterocolitica-specific total IgG (P0.05) and IgG2a antibody responses. Coexpression of IFN-gamma also improved the proliferative T cell responses upon stimulation with Y-Hsp60. A reduction of the splenic bacterial load as compared with the plasmid encoding Y-Hsp60 only was found for the IFN-gamma coexpressing vector. Thus, coexpression of cytokine genes such as IFN-gamma in DNA vaccination vectors might improve immunity and help to overcome the side effects of standard adjuvants. |
| Databáze: | OpenAIRE |
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