MYBL2 (B-MYB) in Cervical Cancer: Putative Biomarker
| Autor: | Hayes Brian, Lynda McEvoy, Cara Martin, Cathy Spillane, John J. O'Leary, Katharine Astbury, Orla Sheils |
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| Rok vydání: | 2011 |
| Předmět: |
Cellular differentiation
Uterine Cervical Neoplasms Cell Cycle Proteins Cervical intraepithelial neoplasia Proto-Oncogene Mas HeLa Cell Line Tumor Biomarkers Tumor medicine Humans Cervical cancer Cervical Glandular Intraepithelial Neoplasia biology business.industry Gene Expression Profiling Obstetrics and Gynecology Cancer medicine.disease biology.organism_classification Molecular biology Gene expression profiling Oncology Trans-Activators Female business Comparative genomic hybridization |
| Zdroj: | International Journal of Gynecologic Cancer. 21:206-212 |
| ISSN: | 1525-1438 1048-891X |
| DOI: | 10.1097/igc.0b013e318205759f |
| Popis: | Introduction:Cervical cancer is the second most common cancer affecting women worldwide. It is characterized by chromosomal aberrations and alteration in the expression levels of many cell cycle regulatory proteins.MYBL2is a member of theMYBproto-oncogene family that encodes DNA binding proteins. These proteins are involved in cell proliferation and control of cellular differentiation.Materials and Methods:Four established cervical cancer cell lines were examined and compared with normal cervix using gene expression profiling and comparative genomic hybridization, and results were correlated to identify potential novel cervical cancer biomarkers. Results were validated using TaqMan polymerase chain reaction, and the potential role of MYBL2 as a clinical biomarker was then evaluated by immunohistochemistry on 30 tissue samples.Results:MYBL2was found to be overexpressed in the cervical cancer cell lines by gene expression profiling, and this result was confirmed using TaqMan polymerase chain reaction. Analysis of comparative genomic hybridization data indicated that chromosome 20q13.1, which encodes theMYBL2gene, was amplified in the human papillomavirus (HPV) type 16-positive CaSki and SiHa cell lines but not in the HPV-18-positive HeLa or HPV-negative C33A cell lines.Discussion:Although MYBL2 staining was predominantly absent in normal cervical epithelium, strong staining (score of 2 or 3) was identified in all cases of cervical intraepithelial neoplasia, cervical glandular intraepithelial neoplasia, and invasive cancer on immunohistochemistry. In addition, strong staining of a population of diffusely scattered single cells is identified. We postulate that these may represent so-called cancer stem-like cells. |
| Databáze: | OpenAIRE |
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