The purification and characterization of homologous high molecular weight storage proteins from grain of wheat, rye and barley

Autor:	J. M. Field, Peter R. Shewry, J. F. March, Benjamin J. Miflin
Rok vydání:	1982
Předmět:	chemistry.chemical_classification Molecular mass Isoelectric focusing Size-exclusion chromatography food and beverages General Medicine Biology Gluten Amino acid chemistry Biochemistry Genetics Storage protein Proline Agronomy and Crop Science Polyacrylamide gel electrophoresis Biotechnology
Zdroj:	Theoretical and Applied Genetics. 62:329-336
ISSN:	1432-2242 0040-5752
DOI:	10.1007/bf00275097
Popis:	Homologous high molecular weight storage prolamins were purified from grain of wheat, rye and barley using combinations of gel filtration, ion-exchange chromatography and preparative isoelectric focusing. Sodium dodecylsulphate polyacrylamide gel electrophoresis showed that the components were single bands with apparent mol.wts. of above 100,000. Molecular weights determined by sedimentation equilibrium ultracentrifugation were considerably lower; 54,700, 67,600 and 69,600 for the components from barley, rye and wheat respectively. Amino acid analysis showed the presence of 13.6 to 16.5 mol% glycine, 29.6 to 34.0 mol% glutamate + glutamine, 11.4 to 13.7 mol% proline and a total of 4.0 to 5.7 mol% basic amino acids. Automated N-terminal amino acid sequencing of the component from wheat showed the presence of cysteine residues at positions 5 and 10, and this is discussed in relation to the possible role of these proteins in the visco-elastic gluten network.
Databáze:	OpenAIRE
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