Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies
| Autor: | Cheikh Sokhna, F Diallo Agne, Souleymane Doucoure, Aziz Hichami, Abdoulaye Ba, Naim Akhtar Khan, Hubert Bassene, Abdoulaye Samb, A Seck |
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| Přispěvatelé: | Cheikh Anta Diop University, Université Bourgogne Franche-Comté [COMUE] (UBFC), Equipe NuTox (LNC - U1231) (NUTOX), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Environnement, Santé, Sociétés (ESS), Centre National de la Recherche Scientifique (CNRS), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Blood Glucose Endocrinology Diabetes and Metabolism medicine.medical_treatment T cell differentiation lcsh:Diseases of the endocrine glands. Clinical endocrinology Gestational diabetes mellitus Fetal Macrosomia chemistry.chemical_compound 0302 clinical medicine Endocrinology Pregnancy [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Insulin GATA3 Cell Differentiation Inflammatory cytokines 3. Good health Gestational diabetes Cholesterol Cytokines Female Research Article Adult medicine.medical_specialty Article Subject 030209 endocrinology & metabolism Proinflammatory cytokine 03 medical and health sciences Th2 Cells Internal medicine Diabetes mellitus medicine Humans Triglycerides Glycated Hemoglobin lcsh:RC648-665 Triglyceride business.industry Infant Newborn Th1 Cells medicine.disease Diabetes Gestational 030104 developmental biology chemistry business |
| Zdroj: | JOURNAL OF DIABETES RESEARCH JOURNAL OF DIABETES RESEARCH, 2018, pp.8474617. ⟨10.1155/2018/8474617⟩ Journal of Diabetes Research Journal of Diabetes Research, Vol 2018 (2018) |
| DOI: | 10.1155/2018/8474617⟩ |
| Popis: | International audience; The aim of the study was to assess T cell differentiation and the modulation of inflammatory cytokines in obese and gestational diabetes mellitus (GDM) women and their macrosomic newborns. Hence, immediately after delivery, blood samples were collected through the mother's arm vein and the umbilical cordon vein. Biochemical parameters measured were HbA1C, glucose, insulin, triglyceride (TG), total cholesterol (Tchol), HDL cholesterol (HDLchol), and LDL cholesterol (LDLchol). T lymphocytes were purified from the total blood with Ficoll-Paque. The mRNA expression of inflammatory markers in T cells was determined by RT-qPCR. We observed that diabetic mothers exhibited higher HbA1C, glycemia, insulinemia, TG, Tchol, HDLchol, and LDLchol levels than control mothers. Glycemia was not significantly different between macrosomic and control newborns. However, insulinemia was high in macrosomic babies. TG, Tchol, HDLchol, and LDLchol were not significantly different between macrosomic and control babies. In diabetic mothers, mRNA expression of the Th1 cell subtype was significantly increased. Th1 markers were upregulated in babies born to diabetic women than in control newborns. However, expression of two Th2 markers (GATA3 and IL-4) was not significantly different between control and GDM women and between their respective newborns. Interestingly, IL-10 mRNA expression was decreased in diabetic mothers and their offsprings. The Th1/Th2 cytokine ratio was increased in GDM obese mothers and their macrosomic newborns, suggesting a proinflammatory status in these subjects. |
| Databáze: | OpenAIRE |
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