Microglia provide structural resolution to injured dendrites after severe seizures
| Autor: | Sruchika Sabu, Ukpong B. Eyo, Mingshu Mo, Long Jun Wu, Koichiro Haruwaka, Lingxiao Wang, Antony Brayan Campos-Salazar, Pingyi Xu, Xenophon S. Speros |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Kainic acid QH301-705.5 dendritic beading Neuronal excitation phagocytic cups Neuroprotection General Biochemistry Genetics and Molecular Biology Article Functional networks 03 medical and health sciences chemistry.chemical_compound Epilepsy Mice 0302 clinical medicine In vivo Seizures Medicine Animals Humans In patient Biology (General) Microglia business.industry microglial process pouches Dendrites medicine.disease 030104 developmental biology medicine.anatomical_structure chemistry two-photon imaging business Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Cell reports Cell Reports, Vol 35, Iss 5, Pp 109080-(2021) |
| ISSN: | 2211-1247 |
| Popis: | SUMMARY Although an imbalance between neuronal excitation and inhibition underlies seizures, clinical approaches that target these mechanisms are insufficient in containing seizures in patients with epilepsy, raising the need for alternative approaches. Brain-resident microglia contribute to the development and stability of neuronal structure and functional networks that are perturbed during seizures. However, the extent of microglial contributions in response to seizures in vivo remain to be elucidated. Using two-photon in vivo imaging to visualize microglial dynamics, we show that severe seizures induce formation of microglial process pouches that target but rarely engulf beaded neuronal dendrites. Microglial process pouches are stable for hours, although they often shrink in size. We further find that microglial process pouches are associated with a better structural resolution of beaded dendrites. These findings provide evidence for the structural resolution of injured dendrites by microglia as a form of neuroprotection. In brief Eyo et al. report that microglia form unique spherical structures in an experimental seizure model. Using live imaging, the authors characterize these structure that they call “microglial process pouches” and show that they colocalize with injured neuronal dendrites and are strongly correlated with the structural resolution of these injured dendrites. Graphical Abstract |
| Databáze: | OpenAIRE |
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