Whole transcriptome expression analysis and comparison of two different strains of Plasmodium falciparum using RNA-Seq
| Autor: | Subhash Chandra Parija, Vrushali Pathak, Amrita Bhattacherjee, Kanjaksha Ghosh, Hiasindh Ashmi Antony |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Plasmodium falciparum RNA-Seq Drug resistance Biology Biochemistry DNA sequencing Transcriptome 03 medical and health sciences 0302 clinical medicine Next generation sequencing Data in Brief Genetics Parasite hosting Chloroquine resistance Transcriptomics Gene biology.organism_classification Malaria lcsh:Genetics 030104 developmental biology Trizol Molecular Medicine 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Genomics Data Genomics Data, Vol 8, Iss C, Pp 110-112 (2016) |
| ISSN: | 2213-5960 |
| Popis: | The emergence and distribution of drug resistance in malaria are serious public health concerns in tropical and subtropical regions of the world. However, the molecular mechanism of drug resistance remains unclear. In the present study, we performed a high-throughput RNA-Seq to identify and characterize the differentially expressed genes between the chloroquine (CQ) sensitive (3D7) and resistant (Dd2) strains of Plasmodium falciparum. The parasite cells were cultured in the presence and absence of CQ by in vitro method. Total RNA was isolated from the harvested parasite cells using TRIzol, and RNA-Seq was conducted using an Illumina HiSeq 2500 sequencing platform with paired-end reads and annotated using Tophat. The transcriptome analysis of P. falciparum revealed the expression of ~5000 genes, in which ~60% of the genes have unknown function. Cuffdiff program was used to identify the differentially expressed genes between the CQ-sensitive and resistant strains. Here, we furnish a detailed description of the experimental design, procedure, and analysis of the transcriptome sequencing data, that have been deposited in the National Center for Biotechnology Information (accession nos. PRJNA308455 and GSE77499). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|