Rapid and Sensitive Lateral Flow Immunoassay Method for Procalcitonin (PCT) Based on Time-Resolved Immunochromatography
| Autor: | Cong-Rong Wang, Chun-Mei Xie, Rong-Liang Liang, Weiwen Xu, Xiang-Yang Shao, Xian-Guo Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Calcitonin
Calcitonin Gene-Related Peptide Point-of-Care Systems Coefficient of variation Analytical chemistry 02 engineering and technology time-resolved lcsh:Chemical technology 01 natural sciences Biochemistry Article Chromatography Affinity Procalcitonin Analytical Chemistry procalcitonin (PCT) immunochromatography Medicine lcsh:TP1-1185 Protein Precursors Electrical and Electronic Engineering Instrumentation Chromatography business.industry 010401 analytical chemistry 021001 nanoscience & nanotechnology Serum samples Atomic and Molecular Physics and Optics 0104 chemical sciences 0210 nano-technology business Biomarkers hormones hormone substitutes and hormone antagonists Lateral flow immunoassay |
| Zdroj: | Sensors, Vol 17, Iss 3, p 480 (2017) Sensors; Volume 17; Issue 3; Pages: 480 Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| Popis: | Procalcitonin (PCT) is a current, frequently-used marker for severe bacterial infection. The aim of this study was to develop a cost-effective detection kit for rapid quantitative and on-site detection of PCT. To develop the new PCT quantitative detecting kit, a double-antibody sandwich immunofluorescent assay was employed based on time-resolved immunofluorescent assay (TRFIA) combined with lateral flow immunoassay (LFIA). The performance of the new developed kit was evaluated in the aspects of linearity, precision, accuracy, and specificity. Two-hundred thirty-four serum samples were enrolled to carry out the comparison test. The new PCT quantitative detecting kit exhibited a higher sensitivity (0.08 ng/mL). The inter-assay coefficient of variation (CV) and the intra-assay CV were 5.4%–7.7% and 5.7%–13.4%, respectively. The recovery rates ranged from 93% to 105%. Furthermore, a high correlation (n = 234, r = 0.977, p < 0.0001) and consistency (Kappa = 0.875) were obtained when compared with the PCT kit from Roche Elecsys BRAHMS. Thus, the new quantitative method for detecting PCT has been successfully established. The results indicated that the newly-developed system based on TRFIA combined with LFIA was suitable for rapid and on-site detection for PCT, which might be a useful platform for other biomarkers in point-of-care tests. |
| Databáze: | OpenAIRE |
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