Identification of an amino acid domain encoded by the capsid gene of porcine circovirus type 2 that modulates intracellular viral protein distribution during replication
| Autor: | Andrew K. Cheung, Justin J. Greenlee |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Circovirus
Cancer Research Swine Viral protein Amino Acid Motifs Kidney Virus Replication medicine.disease_cause Virus Cell Line Viral Proteins Virology medicine Animals Gene Cell Nucleus chemistry.chemical_classification biology biology.organism_classification Molecular biology Amino acid Porcine circovirus Infectious Diseases medicine.anatomical_structure Capsid chemistry Biochemistry Capsid Proteins Nucleus Intracellular |
| Zdroj: | Virus Research. 155:358-362 |
| ISSN: | 0168-1702 |
| Popis: | Previous work showed that distinct amino acid motifs are encoded by the Rep, Cap and ORF3 genes of two subgroups of porcine circoviruses (PCV), PCV2a and PCV2b. At a specific location of the gene, a certain amino acid residue or sequence is preferred. Specifically, two amino acid domains located in the capsid protein, designated motif-1 (six residues located at positions 86-91) and motif-2 (four residues at positions 190-191-206-210), have been identified to associate with either PCV2a (motif-1a:T(N)KI(S)I and motif-2a:SRKD) or PCV2b (motif-1b:S(N)PR(S)V and motif-2b:AGIE) preferentially. In this study, the protein distribution pattern of a PCV2a isolate and a PCV2b isolate was examined. Each virus exhibited a different viral protein pattern during replication in porcine kidney cells and the viral protein distribution pattern was associated with amino acid motif-2 but not motif-1. The results also showed that a more robust accumulation of viral proteins in the nucleus was associated with motif-2b than with motif-2a. In addition, viruses containing motif-2b replicated better than viruses containing motif-2a in porcine kidney cells. |
| Databáze: | OpenAIRE |
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